ArticleNucleic acids research2021
A comprehensive epigenome atlas reveals DNA methylation regulating skeletal muscle development.
Article in Nucleic acids research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers.
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Who cites it
94 citing papers in PubMed, 137 citations in OpenAlex.
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- Genome-wide DNA methylation analysis in pigs using long-read sequencing unveils high-altitude adaptation and allele-specific regulation.BMC genomics · 2026Article
- Identification of differentially expressed lncRNAs in different daily weight gains of Jiangquan black pigs and functional analysis of LOC100518120.BMC genomics · 2026Article
- Multi-Omics Insights Into the Mechanisms of Early Muscle Fiber Difference and Transformation Between Lean-Type and Chinese Indigenous Pigs.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Integrative multi-omics analysis identifies GNAZ and SLC5A4 as critical regulators of gestation length in Large White pigs.Genetics, selection, evolution : GSE · 2026Article
- Whole genome DNA methylation atlas of the scallop Patinopecten yessoensis.Scientific data · 2026Article
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- Dynamic development of muscle during embryogenesis in Arbor Acres broilers and TaoYuan chickens revealed by RNA sequencing.BMC genomics · 2026Article
- Analysis of RNA Expression Specificity and Commonality in Commonly Used Tool Cells and Multiple Tissues of Pigs.Biomolecules · 2026Article
- HMGB2-RAD21 Axis Promotes Fibro/Adipogenic Progenitor Proliferation and Regulates Fat Infiltration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Butyrate supplementation in sows promotes offspring embryonic muscle development by upregulating HDAC-mediated H3K27ac levels and myogenic gene expression.Science China. Life sciences · 2026Article
- Association Between Abnormal DNA Methylation and Altered Transcriptome in Muscle Five Years After Critical Illness.Journal of cachexia, sarcopenia and muscle · 2026Article
- Integrated analysis of DNA methylome and transcriptome of the backfat and longissimus dorsi muscle of Chinese-European hybrid pigs.BMC genomics · 2026Article
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- Comprehensive methylome and transcriptome profiling reveals specific biomarkers for bovine viral diarrhea virus persistent infection in calves.Frontiers in immunology · 2026Article
- Review
- Skeletal muscle methylome-transcriptome disruptions during the onset and progression of colorectal cancer-induced cachexia.American journal of physiology. Cell physiology · 2026Article
- Gut microbiota-mediated betaine regulates skeletal muscle fiber type transition by affecting mGut microbes · 2025Article
- Article
34 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA methylation is important for the epigenetic regulation of gene expression and plays a critical role in mammalian development. However, the dynamic regulation of genome-wide DNA methylation in skeletal muscle development remains largely unknown. Here, we generated the first single-base resolution DNA methylome and transcriptome maps of porcine skeletal muscle across 27 developmental stages. The overall methylation level decreased from the embryo to the adult, which was highly correlated with the downregulated expression of DNMT1 and an increase in partially methylated domains. Notably, we identified over 40 000 developmentally differentially methylated CpGs (dDMCs) that reconstitute the developmental trajectory of skeletal muscle and associate with muscle developmental genes and transcription factors (TFs). The dDMCs were significantly under-represented in promoter regulatory regions but strongly enriched as enhancer histone markers and in chromatin-accessible regions. Integrative analysis revealed the negative regulation of both promoter and gene body methylation in genes associated with muscle contraction and insulin signaling during skeletal muscle development. Mechanistically, DNA methylation affected the expression of muscle-related genes by modulating the accessibly of upstream myogenesis TF binding, indicating the involvement of the DNA methylation/SP1/IGF2BP3 axis in skeletal myogenesis. Our results highlight the function and regulation of dynamic DNA methylation in skeletal muscle development.
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