ArticleNucleic acids research2021
Insights into synthesis and function of KsgA/Dim1-dependent rRNA modifications in archaea.
Article in Nucleic acids research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 28 citations in OpenAlex.
- Proteome characterization of single gene deletion mutants lacking zinc finger µ-proteins inmicroLife · 2026Article
- In Vivo RNA Chemical Footprinting Analysis in Archaea.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Review
- Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.Nature communications · 2025Article
- RNase W, a conserved ribonuclease family with a novel active site.Nucleic acids research · 2024Article
- CNature communications · 2024Article
- The chromatin landscape of the euryarchaeon Haloferax volcanii.Genome biology · 2023Article
- Nanopore-based RNA sequencing deciphers the formation, processing, and modification steps of rRNA intermediates in archaea.RNA (New York, N.Y.) · 2023Article
- Article
- Growth temperature and chromatinization in archaea.Nature microbiology · 2022Article
- Looking through the Lens of the Ribosome Biogenesis Evolutionary History: Possible Implications for Archaeal Phylogeny and Eukaryogenesis.Molecular biology and evolution · 2022Article
- Archaea: A Goldmine for Molecular Biologists and Evolutionists.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Differential Translation Activity Analysis Using Bioorthogonal Noncanonical Amino Acid Tagging (BONCAT) in Archaea.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A Comparative Perspective on Ribosome Biogenesis: Unity and Diversity Across the Tree of Life.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Proteomic Sample Preparation and Data Analysis in Line with the Archaeal Proteome Project.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Tracing Eukaryotic Ribosome Biogenesis Factors Into the Archaeal Domain Sheds Light on the Evolution of Functional Complexity.Frontiers in microbiology · 2021Article
- Ribosome Biogenesis in Archaea.Frontiers in microbiology · 2021Review
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Authors and funding
10 authors at 3 institutions in 2 countries.
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Abstract
Ribosomes are intricate molecular machines ensuring proper protein synthesis in every cell. Ribosome biogenesis is a complex process which has been intensively analyzed in bacteria and eukaryotes. In contrast, our understanding of the in vivo archaeal ribosome biogenesis pathway remains less characterized. Here, we have analyzed the in vivo role of the almost universally conserved ribosomal RNA dimethyltransferase KsgA/Dim1 homolog in archaea. Our study reveals that KsgA/Dim1-dependent 16S rRNA dimethylation is dispensable for the cellular growth of phylogenetically distant archaea. However, proteomics and functional analyses suggest that archaeal KsgA/Dim1 and its rRNA modification activity (i) influence the expression of a subset of proteins and (ii) contribute to archaeal cellular fitness and adaptation. In addition, our study reveals an unexpected KsgA/Dim1-dependent variability of rRNA modifications within the archaeal phylum. Combining structure-based functional studies across evolutionary divergent organisms, we provide evidence on how rRNA structure sequence variability (re-)shapes the KsgA/Dim1-dependent rRNA modification status. Finally, our results suggest an uncoupling between the KsgA/Dim1-dependent rRNA modification completion and its release from the nascent small ribosomal subunit. Collectively, our study provides additional understandings into principles of molecular functional adaptation, and further evolutionary and mechanistic insights into an almost universally conserved step of ribosome synthesis.
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