ArticlePloS one2021
A new analysis method for evaluating bacterial growth with microplate readers.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed.
- EngineeringBiomolecules · 2026Article
- The IrlS2-IrlR2 two-component system is a global regulator of biofilm formation, stress adaptation, and virulence inmSphere · 2026Article
- Dual-function, Reusable, and Flexible Thermal Interface for Kinetic Monitoring of In Vitro Bioassays.Small methods · 2026Article
- Characterize Oral-to-Blood Microbial DNA Translocation in Individuals with Cocaine Use Disorder.bioRxiv : the preprint server for biology · 2026Article
- Fitness costs and persistence of plasmid-mediated cephalosporin resistance inFrontiers in microbiology · 2026Review
- Improvement of isolation and drug testing methods for Corynebacterium spp. in clinical specimens of granulomatous mastitis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025Article
- Concentration-dependent responses of C. reinhardtii to silver ions: hormetic response in growth and reduction of motility.The European physical journal. E, Soft matter · 2025Article
- In vitro phytochemical, antioxidant activity and antimycobacterial potentials of selected medicinal plants commonly used for respiratory infections and related symptoms in the Limpopo Province, South Africa.BMC complementary medicine and therapies · 2025Article
- Type I-E* CRISPR-Cas ofmSphere · 2025Article
- Rapid and accurate testing for urinary tract infection: new clothes for the emperor.Clinical microbiology reviews · 2025Review
- Comparative Analysis of Chemical Composition and Antibacterial Activity of Essential Oils from Five Varieties ofMolecules (Basel, Switzerland) · 2025Article
- AgrC biotinylation inhibits Staphylococcus aureus infection.PloS one · 2025Article
- Whole-Genome analysis ofAIMS microbiology · 2025Article
- Optimizing Production Conditions of a Caspian SeaInternational journal of molecular and cellular medicine · 2025Article
- A dual-inducible control system for multistep biosynthetic pathways.Journal of biological engineering · 2024Article
- Virulome and phylogenomic profiling of a novel Burkholderia pseudomallei strain from an Indian clinical isolate.Molecular genetics and genomics : MGG · 2024Article
- Determination of bisphosphonate properties in terms of bioavailability, bone affinity, and cytotoxicity.Pharmacological reports : PR · 2024Article
- Analysis of Cholera Risk in India: Insights from 2017-18 Serosurvey Data Integrated with Epidemiologic data and Societal Determinants from 2015-2019.PLoS neglected tropical diseases · 2024Article
- Novel delivery systems for controlled release of bacterial therapeutics.Trends in biotechnology · 2024Review
- Cultivating efficiency: high-throughput growth analysis of anaerobic bacteria in compact microplate readers.Microbiology spectrum · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Growth curve measurements are commonly used in microbiology, while the use of microplate readers for such measurements provides better temporal resolution and higher throughput. However, evaluating bacterial growth with microplate readers has been hurdled by barriers such as multiple scattering. Here, we report our development of a method based on the time derivatives of the optical density (OD) and/or fluorescence (FL) of bacterial cultures to overcome these barriers. First, we illustrated our method using quantitative models and numerical simulations, which predicted the number of bacteria and the number of fluorescent proteins in time as well as their time derivatives. Then, we systematically investigated how the time derivatives depend on the parameters in the models/simulations, providing a framework for understanding the FL growth curves. In addition, as a demonstration, we applied our method to study the lag time elongation of bacteria subjected to treatment with silver (Ag+) ions and found that the results from our method corroborated well with that from growth curve fitting by the Gompertz model that has been commonly used in the literature. Furthermore, this method was applied to the growth of bacteria in the presence of silver nanoparticles (AgNPs) at various concentrations, where the OD curve measurements failed. We showed that our method allowed us to successfully extract the growth behavior of the bacteria from the FL measurements and understand how the growth was affected by the AgNPs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.