ArticleAnalytical chemistry2021
Compensation of Strong Water Absorption in Infrared Spectroscopy Reveals the Secondary Structure of Proteins in Dilute Solutions.
Article in Analytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- In Vitro Evaluation of the Antifungal Activity ofPlants (Basel, Switzerland) · 2025Article
- Solvent Exclusion Effect on Infrared Absorption Spectroscopy.Analytical chemistry · 2025Article
- Safe-by-Design Strategies for Intranasal Drug Delivery Systems: Machine and Deep Learning Solutions to Differentiate Epithelial Tissues via Attenuated Total Reflection Fourier Transform Infrared Spectroscopy.ACS pharmacology & translational science · 2025Article
- Benchtop IR Imaging of Live Cells: Monitoring the Total Mass of Biomolecules in Single Cells.Analytical chemistry · 2024Article
- Single-detector double-beam modulation for high-sensitivity infrared spectroscopy.Scientific reports · 2023Article
- Enhanced surface nanoanalytics of transient biomolecular processes.Science advances · 2023Article
- Application of Quantum Cascade Laser-Infrared Spectroscopy and Chemometrics for In-Line Discrimination of Coeluting Proteins from Preparative Size Exclusion Chromatography.Analytical chemistry · 2022Article
- QCL-IR Spectroscopy for In-Line Monitoring of Proteins from Preparative Ion-Exchange Chromatography.Analytical chemistry · 2022Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Infrared (IR) absorption spectroscopy is a powerful tool that can quantify complex biomolecules and their structural conformations. However, conventional approaches to protein analysis in aqueous solutions have been significantly challenged because the strong IR absorption of water overwhelms the limited dynamic range of the detection system and thus allows only a very short path length and a limited concentration sensitivity. Here, we demonstrate a solvent absorption compensation (SAC) approach that can improve the concentration sensitivity and extend the available path length by distinguishing the analyte signal over the full dynamic range at each wavelength. Absorption spectra without any postprocessing show good linearity from 100 to 0.1 mg/mL protein concentration, allowing a >100 times enhanced signal-to-noise ratio in the amide I band compared to the non-SAC results. We apply this method to
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