ArticleNature biotechnology2021
Volumetric live cell imaging with three-dimensional parallelized RESOLFT microscopy.
Article in Nature biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 71 citations in OpenAlex.
- Time-lapse RESOLFT nanoscopy empowered by deep learning.Nature communications · 2026Article
- Quantifying the spatio-temporal image degradation under motion blur in fluorescence microscopy.Biophysical reports · 2026Article
- A versatile nanobody platform for live and super-resolution imaging of synaptic vesicle dynamics and plasticity in rodent and human neurons.Journal of nanobiotechnology · 2026Article
- A Versatile Tool to Predict and Guide RESOLFT Images Based on Photoswitching, Labelling and Optical Properties.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
- Quantitative optical nanoscopy of mitochondrial-derived vesicles in neurons classifies pre-peroxisomal and clearing organelles.Nature communications · 2026Article
- The near-infrared bacteriophytochrome-derived fluorescent protein PENELOPE enables RESOLFT superresolution microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- All-optical strategies to minimize photobleaching in reversibly switchable fluorescent proteins.Nature communications · 2025Article
- Two-dimensional nonlinear structured illumination microscopy with rsEGFP2.Biomedical optics express · 2025Article
- Live STED imaging of functional neuroanatomy.Nature protocols · 2025Review
- Advancing Multicolor Super-Resolution Volume Imaging: Illuminating Complex Cellular Dynamics.JACS Au · 2025Review
- Imaging 3D cell cultures with optical microscopy.Nature methods · 2025Review
- System- and sample-agnostic isotropic three-dimensional microscopy by weakly physics-informed, domain-shift-resistant axial deblurring.Nature communications · 2025Article
- Individual-based modeling unravels spatial and social interactions in bacterial communities.The ISME journal · 2025Review
- Approaching maximum resolution in structured illumination microscopy via accurate noise modeling.Npj imaging · 2025Article
- Fortunate molecules boost signal to background ratio and localization precision in correlation based single molecule localization microscopy.Communications biology · 2024Article
- Approaching Maximum Resolution in Structured Illumination Microscopy via Accurate Noise Modeling.bioRxiv : the preprint server for biology · 2024Article
- Live-cell imaging powered by computation.Nature reviews. Molecular cell biology · 2024Review
- Advanced 3D imaging and organoid bioprinting for biomedical research and therapeutic applications.Advanced drug delivery reviews · 2024Review
- Multicolor Long-Term Single-Particle Tracking Using 10 nm Upconverting Nanoparticles.Nano letters · 2024Article
- Pushing the Resolution Limit of Stimulated Emission Depletion Optical Nanoscopy.International journal of molecular sciences · 2023Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Elucidating the volumetric architecture of organelles and molecules inside cells requires microscopy methods with a sufficiently high spatial resolution in all three dimensions. Current methods are limited by insufficient resolving power along the optical axis, long recording times and photobleaching when applied to live cell imaging. Here, we present a 3D, parallelized, reversible, saturable/switchable optical fluorescence transition (3D pRESOLFT) microscope capable of delivering sub-80-nm 3D resolution in whole living cells. We achieved rapid (1-2 Hz) acquisition of large fields of view (~40 × 40 µm
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.