ArticleScientific reports2021
Metagenomic analysis of formalin-fixed paraffin-embedded tumor and normal mucosa reveals differences in the microbiome of colorectal cancer patients.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.
- The Application of High-Throughput Technologies for the Study of Microbiome and Cancer.Frontiers in genetics · 2021Pooled it
- The gut microbiome as a biomarker and modifiable risk factor in Lynch Syndrome.Familial cancer · 2026Review
- Article
- Opportunities and Challenges of Multiomics for Discovery and Monitoring of Human Pathogens.Environment & health (Washington, D.C.) · 2025Review
- Gut microbiome in colorectal cancer: metagenomics from bench to bedside.JNCI cancer spectrum · 2025Review
- Article
- Microbiological investigation of pregnancies following vaginal radical trachelectomy using 16S rRNA sequencing of FFPE placental specimens.FEBS open bio · 2024Article
- Fusobacteria alterations are associated with colorectal cancer liver metastasis and a poor prognosis.Oncology letters · 2024Article
- Colorectal Cancer Archaeome: A Metagenomic Exploration, Tunisia.Current issues in molecular biology · 2023Article
- The Local Tumor Microbiome Is Associated with Survival in Late-Stage Colorectal Cancer Patients.Microbiology spectrum · 2023Article
- Potential values of formalin-fixed paraffin-embedded tissues for intratumoral microbiome analysis in breast cancer.Heliyon · 2023Article
- Nanotechnology for colorectal cancer detection and treatment.World journal of gastroenterology · 2022Review
- Compartmentalization of the host microbiome: how tumor microbiota shapes checkpoint immunotherapy outcome and offers therapeutic prospects.Journal for immunotherapy of cancer · 2022Review
- Interaction between Bacteria and the Immune System for Cancer Immunotherapy: The α-GalCer Alliance.International journal of molecular sciences · 2022Article
- Unbiased screen for pathogens in human paraffin-embedded tissue samples by whole genome sequencing and metagenomics.Frontiers in cellular and infection microbiology · 2022Article
- Performance of 16S Metagenomic Profiling in Formalin-Fixed Paraffin-Embedded versus Fresh-Frozen Colorectal Cancer Tissues.Cancers · 2021Article
- Technical challenges regarding the use of formalin-fixed paraffin embedded (FFPE) tissue specimens for the detection of bacterial alterations in colorectal cancer.BMC microbiology · 2021Article
- Colorectal Cancer Stage-Specific Fecal Bacterial Community Fingerprinting of the Taiwanese Population and Underpinning of Potential Taxonomic Biomarkers.Microorganisms · 2021Article
- Spontaneous and Induced Tumors in Germ-Free Animals: A General Review.Medicina (Kaunas, Lithuania) · 2021Review
- Emerging Evidence on the Effects of Dietary Factors on the Gut Microbiome in Colorectal Cancer.Frontiers in nutrition · 2021Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An increased risk of developing colorectal cancer (CRC) and other types of tumor is associated to Lynch syndrome (LS), an inherited condition caused by germline mutations in mismatch repair genes. We selected a cohort of LS patients that had developed CRC and had undergone surgical resection. Formalin-fixed paraffin embedded (FFPE) tissue blocks from matched colorectal and normal mucosa were used for genomic DNA extraction with a commercial kit and sequenced by high-throughput sequencing. A metagenomic approach enabled the taxonomic and functional identification of the microbial community and associated genes detected in the specimens. Slightly lower taxonomic diversity was observed in the tumor compared to the non-tumor tissue. Furthermore, the most remarkable differences between tumors and healthy tissue was the significant increase in the genus Fusobacterium in the former, in particular the species F. nucleatum, as well as Camplylobacter or Bacteroides fragilis, in accordance with previous studies of CRC. However, unlike prior studies, the present work is not based on directed detection by qPCR but instead uses a metagenomic approach to retrieve the whole bacterial community, and addresses the additional difficulty of using long-term stored FFPE samples.
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