ArticleProceedings of the National Academy of Sciences of the United States of America2021
High-speed compressed-sensing fluorescence lifetime imaging microscopy of live cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- High-fidelity fast fluorescence lifetime imaging by event-based denoising.Nature biotechnology · 2026Article
- Fluorescence-lifetime optical electrophysiology in contracting cardiomyocytes.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Fast volumetric fluorescence lifetime imaging of multicellular systems using single-objective light-sheet microscopy.Communications biology · 2025Article
- Transition metal complexes as optical probes for super-resolution microscopy.Nature reviews. Chemistry · 2025Review
- Article
- Microscopy-based techniques for studying the material properties of biomolecular condensates in the cellular environment.Biophysical reviews · 2025Review
- High-speed fluorescence lifetime imaging microscopy: techniques, applications, and prospects.Biophotonics discovery · 2025Review
- Compressive streak microscopy for fast sampling of fluorescent reporters of neural activity.Neurophotonics · 2025Article
- Comparative Insights into Photosynthetic, Biochemical, and Ultrastructural Mechanisms in Hibiscus and Pelargonium Plants.Plants (Basel, Switzerland) · 2024Article
- Developing 1,4-Diethyl-1,2,3,4-tetrahydroquinoxalin-substituted Fluorogens Based on GFP Chromophore for Endoplasmic Reticulum and Lysosome Staining.International journal of molecular sciences · 2024Article
- High-throughput fluorescence lifetime imaging flow cytometry.Nature communications · 2024Article
- EventLFM: event camera integrated Fourier light field microscopy for ultrafast 3D imaging.Light, science & applications · 2024Article
- Advancements in fluorescence lifetime imaging microscopy Instrumentation: Towards high speed and 3D.Current opinion in solid state & materials science · 2024Article
- High-speed optical imaging with sCMOS pixel reassignment.Nature communications · 2024Article
- From Cell Populations to Molecular Complexes: Multiplexed Multimodal Microscopy to Explore p53-53BP1 Molecular Interaction.International journal of molecular sciences · 2024Article
- Introducing the Special Issue Honoring Lihong V. Wang, Pioneer in Biomedical Optics.Journal of biomedical optics · 2024Article
- Tutorial on compressed ultrafast photography.Journal of biomedical optics · 2024Review
- Near-Infrared Fluorescence Imaging in Preclinical Models of Glioblastoma.Journal of imaging · 2023Article
- Spatial resolution enhancement in photon-starved STED imaging using deep learning-based fluorescence lifetime analysis.Nanoscale · 2023Article
- Single-shot time-folded fluorescence lifetime imaging.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
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Authors and funding
4 authors.
Funding
Abstract
We present high-resolution, high-speed fluorescence lifetime imaging microscopy (FLIM) of live cells based on a compressed sensing scheme. By leveraging the compressibility of biological scenes in a specific domain, we simultaneously record the time-lapse fluorescence decay upon pulsed laser excitation within a large field of view. The resultant system, referred to as compressed FLIM, can acquire a widefield fluorescence lifetime image within a single camera exposure, eliminating the motion artifact and minimizing the photobleaching and phototoxicity. The imaging speed, limited only by the readout speed of the camera, is up to 100 Hz. We demonstrated the utility of compressed FLIM in imaging various transient dynamics at the microscopic scale.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.