ReviewThe Journal of neuroscience : the official journal of the Society for Neuroscience2021
Patch-seq: Past, Present, and Future.
Review in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 71 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
71 citing papers in PubMed.
- Multi-omics analysis of ion channels expressed by chicken embryo vestibular type I and type II hair cells.Experimental physiology · 2026Article
- Targeting tumour-neuron synapses with intracavitary RNA delivery prevents glioblastoma recurrence.Nature biomedical engineering · 2026Article
- Aspiration Patch Proteomics Enables CE-ESI-MS Proteotyping of Identified Single Neurons in Intact Brain Tissue.Analytical chemistry · 2026Article
- Mouse to Human Cross-Species Transfer Learning for Electrophysiology-to-Transcriptomics Mapping in Cortical GABAergic Interneurons.Neuroinformatics · 2026Article
- Compound models and Pearson residuals for single-cell RNA-seq data without UMIs.Genome biology · 2026Article
- Patch-Clamp Single-Cell Proteomics in Acute Brain Slices: A Framework for Recording, Retrieval, and Interpretation.ACS chemical neuroscience · 2026Article
- Article
- Decoding Alzheimer's Disease One Cell Class at a Time.Cellular and molecular neurobiology · 2026Review
- Correlative Imaging Platform Linking Taste Cell Function to Molecular Identity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- HCN channels reveal conserved and divergent physiology in supragranular pyramidal neurons in primate species.Communications biology · 2026Article
- Induced Proprioceptor and Low-Threshold Mechanoreceptor Neurons Derived from Human Pluripotent Stem Cells Exhibit Distinct Functional Mechanosensory Properties.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Defining spinal motor neuron subtypes across development: from embryonic specification to postnatal maturation.Frontiers in cellular neuroscience · 2026Review
- Identifying and investigating emergent behaviour in neural systems.Frontiers in neuroscience · 2026Article
- Article
- Patch-Clamp Single-Cell Proteomics in Acute Brain Slices: A Framework for Recording, Retrieval, and Interpretation.bioRxiv : the preprint server for biology · 2025Article
- Linking ion channel gene expression to neuronal firing patterns through a statistical-biophysical model.Patterns (New York, N.Y.) · 2025Article
- Combined statistical-biophysical modeling links ion channel genes to physiology of cortical neuron types.Patterns (New York, N.Y.) · 2025Article
- Profiling lamina specific pyramidal neurons using postmortem human formalin fixed paraffin embedded frontal cortex tissue in combination with digital spatial profiling.Journal of neuroscience methods · 2025Article
- Astrocyte diversity and subtypes: aligning transcriptomics with multimodal perspectives.EMBO reports · 2025Review
- Prelimbic cortical excitatory overdrive and inhibitory underdrive accompany environmental suppression of food seeking.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2025Article
11 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
Abstract
Single-cell transcriptomic approaches are revolutionizing neuroscience. Integrating this wealth of data with morphology and physiology, for the comprehensive study of neuronal biology, requires multiplexing gene expression data with complementary techniques. To meet this need, multiple groups in parallel have developed "Patch-seq," a modification of whole-cell patch-clamp protocols that enables mRNA sequencing of cell contents after electrophysiological recordings from individual neurons and morphologic reconstruction of the same cells. In this review, we first outline the critical technical developments that enabled robust Patch-seq experimental efforts and analytical solutions to interpret the rich multimodal data generated. We then review recent applications of Patch-seq that address novel and long-standing questions in neuroscience. These include the following: (1) targeted study of specific neuronal populations based on their anatomic location, functional properties, lineage, or a combination of these factors; (2) the compilation and integration of multimodal cell type atlases; and (3) the investigation of the molecular basis of morphologic and functional diversity. Finally, we highlight potential opportunities for further technical development and lines of research that may benefit from implementing the Patch-seq technique. As a multimodal approach at the intersection of molecular neurobiology and physiology, Patch-seq is uniquely positioned to directly link gene expression to brain function.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.