ArticleBiotechnology for biofuels2021
A rapid thioacidolysis method for biomass lignin composition and tricin analysis.
Article in Biotechnology for biofuels, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 38 citations in OpenAlex.
- Screening high-quality lignins extracted from biomass to prepare biobased wood adhesives.Nature protocols · 2026Review
- Conserved 4-coumarate 3-hydroxylase/ascorbate peroxidase bifunctionality coordinates lignin deposition and plant growth in Brachypodium and Populus.Plant physiology · 2026Article
- Precursor-Dependent Routing of Aromatic Amino Acids Determines Lignin Structure in Grasses by Sensitivity-Enhanced Solid-State NMR.Journal of the American Chemical Society · 2026Article
- Precursor-Dependent Routing of Aromatic Amino Acids Determines Lignin Structure in Grasses by Sensitivity-Enhanced Solid-State NMR.bioRxiv : the preprint server for biology · 2026Article
- A Role for the Plastidial GPT2 Translocator in the Modulation of Lignin Biosynthesis.Plant, cell & environment · 2025Article
- Article
- Integrated Metabolome and Transcriptome Analysis of Gibberellins Mediated the Circadian Rhythm of Leaf Elongation by Regulating Lignin Synthesis in Maize.International journal of molecular sciences · 2024Article
- Mutating alfalfaFrontiers in plant science · 2024Article
- Comparative Analysis of Transcriptomics and Metabolomics Reveals Defense Mechanisms in Melon Cultivars againstInternational journal of molecular sciences · 2023Article
- Multiplex CRISPR/Cas9-mediated mutagenesis of alfalfa FLOWERING LOCUS Ta1 (MsFTa1) leads to delayed flowering time with improved forage biomass yield and quality.Plant biotechnology journal · 2023Article
- Systematic approaches to C-lignin engineering in Medicago truncatula.Biotechnology for biofuels and bioproducts · 2023Article
- FERONIA and wall-associated kinases coordinate defense induced by lignin modification in plant cell walls.Science advances · 2023Article
- Cell wall response of field grownFrontiers in plant science · 2023Article
- Proteomic and metabolic disturbances in lignin-modified Brachypodium distachyon.The Plant cell · 2022Article
- Developmental changes in lignin composition are driven by both monolignol supply and laccase specificity.Science advances · 2022Article
- A gene-editing/complementation strategy for tissue-specific lignin reduction while preserving biomass yield.Biotechnology for biofuels · 2021Article
- Dynamic Application of High and Low Red:Blue Ratios During Lettuce Development Shifts Growth and Metabolite Allocation.Physiologia plantarumArticle
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5 authors at 2 institutions in 1 country.
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Abstract
backgroundBiomass composition varies from plant to plant and greatly affects biomass utilization. Lignin is a heterogeneous phenolic polymer derived mainly from p-coumaryl, coniferyl, and sinapyl alcohols and makes up to 10-25% of lignocellulosic biomass. Recently, tricin, an O-methylated flavone, was identified as a lignin monomer in many grass species. Tricin may function as a nucleation site for lignification and is advocated as a novel target for lignin engineering to reduce lignin content and improve biomass digestibility in grasses. Thioacidolysis is an analytical method that can be adapted to analyze both lignin monomeric composition and tricin content in the lignin polymer. However, the original thioacidolysis procedure is complex, laborious, and time consuming, making it difficult to be adopted for large-scale screening in biomass research. In this study, a modified, rapid higher throughput thioacidolysis method was developed.
resultsIn combination with gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS), the modified thioacidolysis method can be used to simultaneously characterize the lignin composition and tricin content using 2-5 mg of dry samples. The modified method eliminates the solvent extraction and drastically improves the throughput; 80 samples can be processed in one day per person. Our results indicate that there is no significant difference in the determination of lignin S/G ratio and tricin content between the original and modified methods.
conclusionsA modified thioacidolysis protocol was established. The results demonstrate that the modified method can be used for rapid, high-throughput, and reliable lignin composition and tricin content analyses for screening transgenic plants for cell wall modifications or in large-scale genome-wide association studies (GWAS).
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