ArticleBMC microbiology2021
Development of a real-time quantitative PCR method for detection and quantification of Prevotella copri.
Article in BMC microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 12 citations in OpenAlex.
- Prevotella copri impairs bone mass via osteoclast activation in mice.Molecular and cellular biochemistry · 2026Article
- Insight into the mechanisms of Cd(II) on significantly enhancing rhizobacterial ACC deaminase activity in Cd-tolerant Ensifer morelensis A23T.BMC microbiology · 2026Article
- Comparative metagenomic analysis of diarrheal and non-diarrheal gut microbiome delineating the identification of prospective prognostic markers and probiotics to protect from diarrhea: a brief report.Frontiers in cellular and infection microbiology · 2026Article
- Prevotella copri promotes white adipose browning and ameliorates adiposity.Nature communications · 2025Article
- Gut microbiota-derived isovaleric acid ameliorates influenza virus infection via gut-lung axis.NPJ biofilms and microbiomes · 2025Article
- Abundance of Prevotella copri in gut microbiota is inversely related to a healthy diet in patients with type 2 diabetes.Journal of food and drug analysis · 2023Article
- Correlation between Targeted qPCR Assays and Untargeted DNA Shotgun Metagenomic Sequencing for Assessing the Fecal Microbiota in Dogs.Animals : an open access journal from MDPI · 2023Article
- Evaluation of hypoglycemic effect, safety and immunomodulation of Prevotella copri in mice.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundSince its discovery in 2007, the importance of the human gut bacterium Prevotella copri (P. copri) has been widely recognized with its links to diet and health status and potential as next generation probiotic. Therefore, precise, convenient and cost-effective diagnostic tools for the detection and quantification of P. copri from clinical and environmental samples are needed.
resultsIn this study, a Sybr Green qPCR protocol for P. copri detection and quantification was developed and tested on P. copri-spiked murine faeces samples targeting both the 16S rRNA gene and P. copri genome specific genes. The use of one 16S rRNA primer pair and 2 genome specific primer pairs resulted in at least 10x higher specificity and sensitivity than the primer-only PCR currently cited in the literature, reaching a sensitivity of 10
conclusionsThis improved qPCR-based method will allow accurate P. copri identification and quantification. Moreover, this methodology can also be applied to identify other bacterial species in complex samples.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.