ArticleBiosensors & bioelectronics2021
PfAgo-based detection of SARS-CoV-2.
Article in Biosensors & bioelectronics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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The trial behind it
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Who cites it
45 citing papers in PubMed, 117 citations in OpenAlex.
- Structure-Based Rational Design of TcAgo fromBiomolecules · 2026Article
- Integrating isothermal amplification with magnetic flocculation and DNA-guided PfAgo for SARS-CoV-2 detection.Frontiers in microbiology · 2026Article
- Highly sensitive PfAgo-based biosensor for the detection of mutations in the 23S rRNA gene of macrolide-resistant Mycoplasma pneumoniae.Analytical and bioanalytical chemistry · 2025Article
- An Engineered PfAgo with Wide Catalytic Temperature Range and Substrate Spectrum.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Rapid and Ultrasensitive Detection ofJournal of microbiology and biotechnology · 2025Article
- Visualization of Sex Identification in Red-Crowned Crane (Ecology and evolution · 2025Article
- In vitro programmable DNA cleavage by a eukaryotic Argonaute.Nucleic acids research · 2025Article
- Using Recombinase-Aid Amplification Combined withAnimals : an open access journal from MDPI · 2024Article
- Pyrococcus furiosus Argonaute-mediated porcine epidemic diarrhea virus detection.Applied microbiology and biotechnology · 2024Article
- Rapid and supersensitive allele detection of Plasmodium falciparum chloroquine resistance via a Pyrococcus furiosus argonaute-triggered dual-signal biosensing platform.Parasites & vectors · 2024Article
- A conditional protein diffusion model generates artificial programmable endonuclease sequences with enhanced activity.Cell discovery · 2024Article
- PfAgo-based dual signal amplification biosensor for rapid and highly sensitive detection of alkaline phosphatase activity.Mikrochimica acta · 2024Article
- Highly sensitive detection of a long-COVID-related SNP in LZTFL1 allele withActa biochimica et biophysica Sinica · 2024Article
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- MnChemical science · 2024Article
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- Rapid and sensitive detection of Mycoplasma synoviae using RPA combined with Pyrococcus furiosus Argonaute.Poultry science · 2024Article
- Fast and Ultrasensitive Detection of Monkeypox by aViruses · 2024Article
- New design strategies for ultra-specific CRISPR-Cas13a-based RNA detection with single-nucleotide mismatch sensitivity.Nucleic acids research · 2024Article
- RT-RPA-Frontiers in cellular and infection microbiology · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the present study, we upgraded Pyrococcus furiosus Argonaute (PfAgo) mediated nucleic acid detection method and established a highly sensitive and accurate molecular diagnosis platform for the large-scale screening of COVID-19 infection. Briefly, RT-PCR was performed with the viral RNA extracted from nasopharyngeal or oropharyngeal swabs as template to amplify conserved regions in the viral genome. Next, PfAgo, guide DNAs and molecular beacons in appropriate buffer were added to the PCR products, followed by incubating at 95 °C for 20-30 min. Subsequently, the fluorescence signal was detected. This method was named as SARS-CoV-2 PAND. The whole procedure is accomplished in approximately an hour with the using time of the Real-time fluorescence quantitative PCR instrument shortened from >1 h to only 3-5 min per batch in comparison with RT-qPCR, hence the shortage of the expensive Real-time PCR instrument is alleviated. Moreover, this platform was also applied to identify SARS-CoV-2 D614G mutant due to its single-nucleotide specificity. The diagnostic results of clinic samples with SARS-CoV-2 PAND displayed 100% consistence with RT-qPCR test.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.