ArticleThe Science of the total environment2021
Evaluation of two rapid ultrafiltration-based methods for SARS-CoV-2 concentration from wastewater.
Article in The Science of the total environment, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers, 1 of them a synthesis that pooled it.
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Who cites it
55 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Factors influencing SARS-CoV-2 RNA concentrations in wastewater up to the sampling stage: A systematic review.The Science of the total environment · 2022Pooled it
- Integrated monitoring of enveloped viruses in hospital environments: Detection, persistence, and implications for infection control.PloS one · 2026Article
- Recovering wastewater RNA for virome sequencing by systematically optimized tangential-flow ultrafiltration and Nanotrap microbiome particles.Applied and environmental microbiology · 2025Article
- Long-Term Sewage Survey of SARS-CoV-2, Influenza A and Respiratory Syncytial Virus (RSV), and Correlation to Human Cases in a City with One Million Inhabitants.Microorganisms · 2025Article
- Benchmarking Concentration and Extraction Methods for Wastewater-Based Surveillance of Eight Human Respiratory Viruses: Implications for Rapid Application to Novel Pathogens.Environmental science & technology · 2025Article
- Comparative Efficiency of High-Throughput Magnetic Bead Method and Polyethylene Glycol Precipitation for Viral Concentration in Sewage.Food and environmental virology · 2025Article
- Trends and Challenges in the Detection and Environmental Surveillance of the Hepatitis E Virus.Microorganisms · 2025Review
- Hydrological and physicochemical parameters associated with SARS-CoV-2 and pepper mild mottle virus wastewater concentrations for a large-combined sewer system.Journal of water and health · 2025Article
- Protein Nanoparticles for Targeted SARS-CoV-2 Trapping and Neutralization.Advanced healthcare materials · 2025Article
- Biosynthesis of Gold Nanostructures and Their Virucidal Activity Against Influenza A Virus.International journal of molecular sciences · 2025Article
- Consistent trends from different methods for monitoring SARS-CoV-2 in urban wastewater during a 29-month longitudinal study.Frontiers in microbiology · 2025Article
- Detection of SARS-CoV-2 in wastewater as an earlier predictor of COVID-19 epidemic peaks in Venezuela.Scientific reports · 2024Article
- A multi-tiered approach to assess fecal pollution in an urban watershed: Bacterial and viral indicators and sediment microbial communities.The Science of the total environment · 2024Article
- Evaluation of an Automated Ultrafiltration System for Concentrating a Range of Viruses from Saline Waters.Food and environmental virology · 2024Article
- Article
- Target enrichment metaviromics enables comprehensive surveillance of coronaviruses in environmental and animal samples.Heliyon · 2024Article
- A scoping review of global SARS-CoV-2 wastewater-based epidemiology in light of COVID-19 pandemic.Heliyon · 2024Article
- Real-Time On-Site Monitoring of Viruses in Wastewater Using NanotrapBiosensors · 2024Article
- Simple SARS-CoV-2 concentration methods for wastewater surveillance in low resource settings.The Science of the total environment · 2024Article
- Surveillance of SARS-CoV-2, rotavirus, norovirus genogroup II, and human adenovirus in wastewater as an epidemiological tool to anticipate outbreaks of COVID-19 and acute gastroenteritis in a city without a wastewater treatment plant in the Peruvian Highlands.The Science of the total environment · 2023Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative measurements of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in raw wastewater have been implemented worldwide since the beginning of the pandemic. Recent efforts are being made to evaluate different viral concentration methodologies to overcome supplier shortages during lockdowns. A set of 22-wastewater samples seeded with murine hepatitis virus (MHV), a member of the Coronaviridae family, and the bacteriophage MS2, were used to characterize and compare two ultrafiltration-based methods: a centrifugal ultrafiltration device (Centricon® Plus-70) and the automated concentrating pipette CP-Select™. Based on the recovery efficiencies, significant differences were observed for MHV, with Centricon® Plus-70 (24%) being the most efficient method. Nevertheless, concentrations of naturally occurring SARS-CoV-2, Human adenoviruses and JC polyomaviruses in these samples did not result in significant differences between methods suggesting that testing naturally occurring viruses may complement the evaluation of viral concentration methodologies. Based on the virus adsorption to solids and the necessity of a pre-centrifugation step to remove larger particles and avoid clogging when using ultrafiltration methods, we assessed the percentage of viruses not quantified after ultrafiltration. Around 23% of the detected SARS-CoV-2 would be discarded during the debris removal step. The CP-Select™ provided the highest concentration factor (up to 333×) and the lowest LoD (6.19 × 10
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