ArticleAngewandte Chemie (International ed. in English)2021
In Vivo Assembly of Artificial Metalloenzymes and Application in Whole-Cell Biocatalysis*.
Article in Angewandte Chemie (International ed. in English), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 90 citations in OpenAlex.
- Intracellular assembly of artificial enzymes for cytoplasmic enantioselective Mannich reactions.Nature communications · 2026Article
- Bioorthogonal Gold-Catalyzed Hydrothiolation Leading to Amide Bond Cleavage of Ethynylated Biarylbutanamide Precursors.Journal of the American Chemical Society · 2026Article
- Atomically Precise Clusterzymes: A Programmable Optoelectronic Platform for Neuroscience.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Article
- Artificial Metalloenzymes in Artificial Sanctuaries Through Liquid-Liquid Phase Separation.Bio-protocol · 2025Article
- Activity-Enhancing Mutations in an LmrR-Based Artificial Metalloenzyme Destabilize the Protein Scaffold and Alter its Conformational Plasticity.Chembiochem : a European journal of chemical biology · 2025Article
- Streamlined Identification of Metallopeptides for Intracellular Catalysis Using Positionally Addressable Combinatorial Libraries.ACS catalysis · 2025Article
- Creating novel metabolic pathways by protein engineering for bioproduction.Trends in biotechnology · 2025Review
- Permeabilisation of the Outer Membrane of Escherichia coli for Enhanced Transport of Complex Molecules.Microbial biotechnology · 2025Article
- De novo design and evolution of an artificial metathase for cytoplasmic olefin metathesis.Nature catalysis · 2025Article
- Genetically Engineered Liposwitch-Based Nanomaterials.Biomacromolecules · 2024Article
- Hybrid Metal Catalysts as Valuable Tools in Organic Synthesis: An Overview of the Recent Advances in Asymmetric C─C Bond Formation Reactions.Molecules (Basel, Switzerland) · 2024Review
- Computation-guided engineering of distal mutations in an artificial enzyme.Faraday discussions · 2024Article
- Nanoscale and chiral metal-organic frameworks for asymmetric reactions in water: bridging Lewis acid catalysis and biological systems.Chemical science · 2024Article
- Discovery and application of food catalysts to promote the coupling of PQQ (quinone) with amines.Frontiers in nutrition · 2024Article
- A high-field cellular DNP-supported solid-state NMR approach to study proteins with sub-cellular specificity.Chemical science · 2023Article
- Biodegradable Grubbs-Loaded Artificial Organelles for Endosomal Ring-Closing Metathesis.Biomacromolecules · 2023Article
- First and second sphere interactions accelerate non-nativeChemical communications (Cambridge, England) · 2023Article
- Dual-Bioorthogonal Catalysis by a Palladium Peptide Complex.Journal of medicinal chemistry · 2023Article
- Probing Cell-Surface Interactions in Fungal Cell Walls by High-ResolutionChemistry (Weinheim an der Bergstrasse, Germany) · 2023Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report the supramolecular assembly of artificial metalloenzymes (ArMs), based on the Lactococcal multidrug resistance regulator (LmrR) and an exogeneous copper(II)-phenanthroline complex, in the cytoplasm of E. coli cells. A combination of catalysis, cell-fractionation, and inhibitor experiments, supplemented with in-cell solid-state NMR spectroscopy, confirmed the in-cell assembly. The ArM-containing whole cells were active in the catalysis of the enantioselective Friedel-Crafts alkylation of indoles and the Diels-Alder reaction of azachalcone with cyclopentadiene. Directed evolution resulted in two different improved mutants for both reactions, LmrR_A92E_M8D and LmrR_A92E_V15A, respectively. The whole-cell ArM system required no engineering of the microbial host, the protein scaffold, or the cofactor to achieve ArM assembly and catalysis. We consider this a key step towards integrating abiological catalysis with biosynthesis to generate a hybrid metabolism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.