ArticleJournal, genetic engineering & biotechnology2021
Assessment of genetic diversity among Iranian Aegilops triuncialis accessions using ISSR, SCoT, and CBDP markers.
Article in Journal, genetic engineering & biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 63 citations in OpenAlex.
- Chloroplast Genome Characterization, Comparative Analysis, and Phylogenetic Insights into FiveInternational journal of molecular sciences · 2026Article
- Start codon targeted (SCoT) based molecular diversity and population structure analysis in black turmeric (Curcuma caesia Roxb.).BMC plant biology · 2026Article
- Genetic diversity of native Cucumis melo L. accessions from Iran and Iraq revealed by SCoT and ISSR markers.Scientific reports · 2025Article
- Effect of gamma radiation on in vitro morphogenesis, anatomy and DNA polymorphism of Moringa concanensis Nimmo.Scientific reports · 2025Article
- Genetic diversity and population structure analysis of Indian blackberry (Syzygium cumini L.) using CAAT box‑derived polymorphism (CBDP) and start codon targeted polymorphism (SCoT) markers.Journal, genetic engineering & biotechnology · 2025Article
- Article
- Development of highly discriminatory SCoT- and CBDP-based SCAR fingerprint for authentication of Indian senna (Frontiers in plant science · 2024Article
- Investigation of genetic diversity of Iranian wild relatives of bread wheat using ISSR and SSR markers.Journal, genetic engineering & biotechnology · 2023Article
- Article
- Cis-element amplified polymorphism (CEAP), a novel promoter- and gene-targeted molecular marker of plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Review
- Molecular characterization of India Ginseng Withania somnifera (L) using ISSR markers.Molecular biology reports · 2021Article
- Molecular diversity analysis in hexaploid wheat (Triticum aestivum L.) and two Aegilops species (Aegilops crassa and Aegilops cylindrica) using CBDP and SCoT markers.Journal, genetic engineering & biotechnology · 2021Article
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4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundCrop wild relatives (CWRs) are commonly used as a suitable genetic reservoir for plant breeding. They can be used for enhancing the tolerance of plant varieties to biotic and abiotic stresses. Studying the genetic diversity of related wheat species in Iran could be useful to improve different traits of bread wheat, since the country is one of the major centers of genetic diversity and distribution of Aegilops species. Therefore, the aim of the present study was to determine the relationship among 48 Aegilops triuncialis accessions using three DNA marker systems, including start codon targeted (SCoT), CAAT box-derived polymorphism (CBDP), and inter-simple sequence repeat (ISSR) markers.
resultsA total of 359 amplified DNA fragments were generated using 13 CBDP, 14 SCoT, and 16 ISSR primers that produced 96, 147, and 152 bands, respectively. The discriminating power of the three markers was assessed using polymorphism information content (PIC), marker index (MI), and resolving power (Rp). The mean values of PIC for ISSR, SCoT, and CBDP markers were 0.3, 0.26, and 0.34, respectively, indicating the efficiency of the three markers in detecting polymorphism among the studied accessions. ISSR markers had the highest values of MI, Rp, and polymorphism percentage as compared to SCoT and CBDP markers. Based on the Shannon index and heterozygosity values, genetic diversity in the Alborz population was more than in other populations. The accessions were classified into six, five, and five groups based on ISSR, SCoT, and CBDP using the UPGMA method. According to the results of cluster and PCoA analyses, the variation patterns corresponded with the geographical distribution of the Ae. triuncialis accessions.
conclusionsThe three markers provided a comprehensive pattern of the genetic diversity among the Iranian Ae. triuncialis accessions. This information could allow for a future insight into wheat breeding programs.
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