ArticleApplied microbiology and biotechnology2021
Metagenomic identification, purification and characterisation of the Bifidobacterium adolescentis BgaC β-galactosidase.
Article in Applied microbiology and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- bifidoAnnotator: fine-grained annotation of bifidobacterial glycoside hydrolases for human milk glycan utilization.Microbial genomics · 2026Article
- Article
- Characterization of a β-Galactosidase fromMolecules (Basel, Switzerland) · 2025Article
- Novel degenerate PCR primers targeting the β-galactosidase gene for broad-spectrum identification of lactose-degrading bacteria.World journal of microbiology & biotechnology · 2025Article
- Immobilized β-galactosidase BgaC from Bifidobacterium adolescentis retains stability and activity during repeated cycles of use.Applied microbiology and biotechnology · 2025Article
- Prebiotic Effects of α- and β-Galactooligosaccharides: The Structure-Function Relation.Molecules (Basel, Switzerland) · 2025Review
- Cross-feeding of bifidobacteria promotes intestinal homeostasis: a lifelong perspective on the host health.NPJ biofilms and microbiomes · 2024Review
- Mechanism of 2'-fucosyllactose degradation by human-associatedJournal of bacteriology · 2024Article
- What happens to Bifidobacterium adolescentis and Bifidobacterium longum ssp. longum in an experimental environment with eukaryotic cells?BMC microbiology · 2024Article
- Supplementation with a cranberry extract favors the establishment of butyrogenic guilds in the human fermentation SHIME system.Microbiome research reports · 2024Article
- Cloning, Expression, Purification, and Characterization of a Novel β-Galactosidase/α-L-Arabinopyranosidase fromMolecules (Basel, Switzerland) · 2023Article
- Recent advances in enzymatic properties, preparation methods, and functions of glycoside hydrolase from Bifidobacterium: a review.World journal of microbiology & biotechnology · 2023Review
- Advances in Low-Lactose/Lactose-Free Dairy Products and Their Production.Foods (Basel, Switzerland) · 2023Review
- Baohe pill decoction treats diarrhea induced by high-fat and high-protein diet by regulating lactase-producing bacteria in intestinal mucosa.Frontiers in microbiology · 2023Article
- Cloning, Expression, Purification, and Characterization of β-Galactosidase fromMolecules (Basel, Switzerland) · 2022Article
- An acid-tolerant and cold-active β-galactosidase potentially suitable to process milk and whey samples.Applied microbiology and biotechnology · 2022Article
- Industrial applications of cold-adapted enzymes: challenges, innovations and future perspective.3 Biotech · 2021Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the human gut microbiota use glycoside hydrolase (GH) enzymes, such as β-galactosidases, to forage on host mucin glycans and dietary fibres. A human faecal metagenomic fosmid library was constructed and functionally screened to identify novel β-galactosidases. Out of the 16,000 clones screened, 30 β-galactosidase-positive clones were identified. The β-galactosidase gene found in the majority of the clones was BAD_1582 from Bifidobacterium adolescentis, subsequently named bgaC. This gene was cloned with a hexahistidine tag, expressed in Escherichia coli and His-tagged-BgaC was purified using Ni
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