Evidence map›Paper›PMID 33427280›Full record

ArticleSoft matter2021

Dynamic light scattering microrheology for soft and living materials.

Pamela C Cai, Brad A Krajina, Michael J Kratochvil, Lei Zou, Audrey Zhu, Elizabeth B Burgener, Paul L Bollyky, Carlos E Milla, Matthew J Webber, Andrew J Spakowitz and 1 more

Abstract read
In one paragraph

Article in Soft matter, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. High-throughput Mucus Microrheology for Phenotyping and Disease Modeling.bioRxiv : the preprint server for biology · 2025
    Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Synthetic living materials in cancer biology.Nature reviews bioengineering · 2023
    Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Pamela C CaiDepartment of Chemical Engineering, Stanford University, Stanford, CA 94305, USA. ajspakow@stanford.edu.
Brad A KrajinaDepartment of Chemical Engineering, Stanford University, Stanford, CA 94305, USA. ajspakow@stanford.edu.
Michael J KratochvilDepartment of Materials Science, Stanford University, Stanford, CA 94305, USA. heilshorn@stanford.edu and Stanford Immunology, Stanford University, Stanford, CA 94305, USA.ORCID http://orcid.org/0000-0002-8845-2524
Lei ZouDepartment of Chemical & Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46556, USA.ORCID http://orcid.org/0000-0001-8247-7184
Audrey ZhuDepartment of Chemical Engineering, Stanford University, Stanford, CA 94305, USA. ajspakow@stanford.edu.
Elizabeth B BurgenerCenter for Excellence in Pulmonary Biology, Department of Pediatrics, Stanford University, Stanford, CA 94305, USA.
Paul L BollykyStanford Immunology, Stanford University, Stanford, CA 94305, USA.
Carlos E MillaCenter for Excellence in Pulmonary Biology, Department of Pediatrics, Stanford University, Stanford, CA 94305, USA.ORCID http://orcid.org/0000-0001-5515-3053
Matthew J WebberDepartment of Chemical & Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46556, USA.ORCID http://orcid.org/0000-0003-3111-6228
Andrew J SpakowitzDepartment of Chemical Engineering, Stanford University, Stanford, CA 94305, USA. ajspakow@stanford.edu and Department of Materials Science, Stanford University, Stanford, CA 94305, USA. heilshorn@stanford.edu and Department of Applied Physics, Stanford University, Stanford, CA 94305, USA and Biophysics Program, Stanford University, Stanford, CA 94305, USA.ORCID http://orcid.org/0000-0002-0585-1942
Sarah C HeilshornDepartment of Materials Science, Stanford University, Stanford, CA 94305, USA. heilshorn@stanford.edu.ORCID http://orcid.org/0000-0002-9801-6304

Funding

Injectable Hydrogels to Deliver Gene Therapy for Myocardial InfarctR01HL151997 · NHLBI · STANFORD UNIVERSITY · PI HEILSHORN, SARAH C · 2020 to 2023
$1.7M
Engineered matrix microarrays to enhance the regenerative potential of iPSC-derived endothelial cellsR01HL142718 · NHLBI · STANFORD UNIVERSITY · PI HEILSHORN, SARAH C, HUANG, NGAN F. · 2018 to 2021
$1.6M
Injectable Hydrogels to Protect Transplanted Cells from HypoxiaR01EB027666 · NIBIB · STANFORD UNIVERSITY · PI HEILSHORN, SARAH C, PLANT, GILES · 2019 to 2022
$1.4M
Engineered biomaterials to modulate cell-cell signaling for the robust expansion of stem cellsR01EB027171 · NIBIB · STANFORD UNIVERSITY · PI HEILSHORN, SARAH C · 2019 to 2022
$1.4M
Engineered Protein Hydrogels to Modulate Adipose-derived Stromal Cell Secretome and Exosomes for Injectable Myocardial Infarction TherapyR21HL138042 · NHLBI · STANFORD UNIVERSITY · PI HEILSHORN, SARAH C · 2017 to 2018
$435k
NHLBI NIH HHS R01 HL142718NHLBI NIH HHS R01 HL151997NHLBI NIH HHS R21 HL138042NIBIB NIH HHS R01 EB027171NIBIB NIH HHS R01 EB027666
6 · The paper itself

Abstract

We present a method for using dynamic light scattering in the single-scattering limit to measure the viscoelastic moduli of soft materials. This microrheology technique only requires a small sample volume of 12 μL to measure up to six decades in time of rheological behavior. We demonstrate the use of dynamic light scattering microrheology (DLSμR) on a variety of soft materials, including dilute polymer solutions, covalently-crosslinked polymer gels, and active, biological fluids. In this work, we detail the procedure for applying the technique to new materials and discuss the critical considerations for implementing the technique, including a custom analysis script for analyzing data output. We focus on the advantages of applying DLSμR to biologically relevant materials: breast cancer cells encapsulated in a collagen gel and cystic fibrosis sputum. DLSμR is an easy, efficient, and economical rheological technique that can guide the design of new polymeric materials and facilitate the understanding of the underlying physics governing behavior of naturally derived materials.

Indexed as

PolymersDynamic Light ScatteringGelsRheologyViscosityGelsPolymers

Identifiers

PMID33427280
PMCPMC7938343

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.