Evidence map›Paper›PMID 33427237›Full record

ArticleJournal of visualized experiments : JoVE2020

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging.

Kelly Veerasammy, Yuki X Chen, Sami Sauma, Mathilde Pruvost, David K Dansu, Tenzin Choetso, Tiffany Zhong, Damien Marechal, Patrizia Casaccia, Rinat Abzalimov and 1 more

Open access · greenAbstract readVideo-Audio Media
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of visualized experiments : JoVE, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.7field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 9 citations in OpenAlex.

  1. PLANCK: super-multiplex optical imaging without labeling.bioRxiv : the preprint server for biology · 2026
    Article
  2. Spatial Molecular Imaging of the Glycome Using Mass Spectrometry.Journal of visualized experiments : JoVE · 2025
    Article
  3. Review
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Kelly Veerasammy *The Graduate Center - Advanced Science Research Center, Neuroscience Initiative, The City University of New York; The City College of New York, CUNY.
Yuki X Chen *The Graduate Center - Advanced Science Research Center, Neuroscience Initiative, The City University of New York; The City College of New York, CUNY.
Sami Sauma *The Graduate Center - Advanced Science Research Center, Neuroscience Initiative, The City University of New York.
Mathilde PruvostThe Graduate Center - Advanced Science Research Center, Neuroscience Initiative, The City University of New York.
David K DansuThe Graduate Center - Advanced Science Research Center, Neuroscience Initiative, The City University of New York.
Tenzin ChoetsoThe Graduate Center - Advanced Science Research Center, Neuroscience Initiative, The City University of New York; The City College of New York, CUNY.
Tiffany ZhongThe Bronx High School of Science.
Damien MarechalThe Graduate Center - Advanced Science Research Center, Neuroscience Initiative, The City University of New York.
Patrizia CasacciaThe Graduate Center - Advanced Science Research Center, Neuroscience Initiative, The City University of New York.
Rinat AbzalimovThe Graduate Center - Advanced Science Research Center, Structural Biology Initiative, The City University of New York; The Graduate Center - Advanced Science Research Center, MALDI MS Imaging Joint Core Facility, The City University of New York; rabzalimov@gc.cuny.edu.
Ye HeThe Graduate Center - Advanced Science Research Center, Neuroscience Initiative, The City University of New York; The Graduate Center - Advanced Science Research Center, MALDI MS Imaging Joint Core Facility, The City University of New York; yhe1@gc.cuny.edu.
The Graduate Center, CUNY · USBronx High School of Science · US

Funding

Environmental Biosensors in the Oligodendrocyte LineageR35NS111604 · NINDS · ADVANCED SCIENCE RESEARCH CENTER · PI Patrizia Casaccia · 2019 to 2026
$9.1M
NINDS NIH HHS R35 NS111604
6 · The paper itself

Abstract

Metabolomics, the study to identify and quantify small molecules and metabolites present in an experimental sample, has emerged as an important tool to investigate the biological activities during development and diseases. Metabolomics approaches are widely employed in the study of cancer, nutrition/diet, diabetes, and other physiological and pathological conditions involving metabolic processes. An advantageous tool that aids in metabolomic profiling advocated in this paper is matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI). Its ability to detect metabolites in situ without labeling, structural modifications, or other specialized reagents, such as those used in immunostaining, makes MALDI MSI a unique tool in advancing methodologies relevant in the field of metabolomics. An appropriate sample preparation process is critical to yield optimal results and will be the focus of this paper.

Indexed as

Analytic Sample Preparation MethodsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationAnimalsCryoultramicrotomyHumansMetabolomicsMice, Inbred C57BL

Identifiers

PMID33427237
PMCPMC11819924
OpenAlexW3115149523

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.