ArticleInternational journal of reproductive biomedicine2020
The effect of the human cumulus cells-conditioned medium on in vitro maturation of mouse oocyte: An experimental study.
Article in International journal of reproductive biomedicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 12 citations in OpenAlex.
- Conditioned media and extracellular vesicles derived from human Wharton's jelly mesenchymal stem cells improve the in vitro maturation of immature oocytes in normal and PCOS mouse model.Biomedical engineering online · 2025Article
- Follicular fluid meiosis activating sterol supplementation enhances oocyte maturation and fertilization in a microfluidic system: A lab trial study.International journal of reproductive biomedicine · 2024Article
- The Effect of The Conditioned Medium from Human Embryonic Stem Cells on Mouse Oocytes In Vitro Maturation.Cell journal · 2023Article
- Quantitative morphokinetic parameters identify novel dynamics of oocyte meiotic maturation and cumulus expansion†.Biology of reproduction · 2022Article
- Effect of endometrial cell-conditioned medium and platelet-rich plasma on the developmental competence of mouse preantral follicles: An in vitro study.Clinical and experimental reproductive medicine · 2022Article
- Mesenchymal stem cells to treat female infertility; future perspective and challenges: A review.International journal of reproductive biomedicine · 2022Review
- Effect of Passage Number of Conditioned Medium Collected from Equine Amniotic Fluid Mesenchymal Stem Cells: Porcine Oocyte Maturation and Embryo Development.International journal of molecular sciences · 2022Article
- Potential of Mesenchymal Stem Cell-Derived Exosomes as a Novel Treatment for Female Infertility Caused by Bacterial Infections.Frontiers in microbiology · 2021Review
- The effect of the human cumulus cells-conditioned medium on in vitro maturation of mouse oocyte: An experimental study.International journal of reproductive biomedicine · 2020Article
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTo increase the results of infertility treatment, many efforts have been made to improve the treatment methods. As assisted reproductive technology is mainly using cell culture methods, one of the approaches to improve this technology is conditioned medium from different sources. It is desirable to apply
objectiveTo investigate the effect of human cumulus cell condition medium (hCCCM) on the IVM of immature mouse oocytes and morphology. MATERIALS AND
methodsIn this experimental study, 240 germinal vesile oocytes were collected from four-six wk-old mice after 48 hr of 5IU pregnant mare serum gonadotropin (PMSG) injection and cultured in hCCCM (test group, n = 120) and DMEM + 20% FBS (control group, n = 120). The IVM rates and changes in perivitelline space (PVS) and shape were investigated at 8, 16, and 24 hr following the culture. The mature (MII) oocytes were subjected to in vitro fertilization (IVF) and the fertilization rate was assessed in three days.
resultsA significant difference was observed between the maturation rates in the hCCCM and control groups (24.16% vs 0%; p = 0.001), as well as morphologic changes between the two groups (p = 0.04, p = 0.05). The development rate for MII oocytes attained from IVM in the hCCCM group was 27.58% (2-cell) and 6.89% (4-cell). Data displayed that hCCCM is an effective medium for oocytes maturation compared to the control medium.
conclusionhCCCM supports oocyte
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