Evidence map›Paper›PMID 33426222›Full record

ArticleRegenerative therapy2020

Responses of oral-microflora-exposed dental pulp to capping with a triple antibiotic paste or calcium hydroxide cement in mouse molars.

Angela Quispe-Salcedo, Takuichi Sato, Junko Matsuyama, Hiroko Ida-Yonemochi, Hayato Ohshima

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Regenerative therapy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Angela Quispe-SalcedoDivision of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, 2-5274 Gakkocho-dori, Chuo-ku, Niigata, 951-8514, Japan.
Takuichi SatoDivision of Clinical Chemistry, Department of Medical Technology, Niigata University Graduate School of Health Sciences, 2-746 Asahimachi-dori, Chuo-ku, Niigata, 951-8518, Japan.
Junko MatsuyamaDivision of Pediatric Dentistry, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences, 2-5274 Gakkocho-dori, Chuo-ku, Niigata, 951-8514, Japan.
Hiroko Ida-YonemochiDivision of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, 2-5274 Gakkocho-dori, Chuo-ku, Niigata, 951-8514, Japan.
Hayato OhshimaDivision of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, 2-5274 Gakkocho-dori, Chuo-ku, Niigata, 951-8514, Japan.
Niigata University · JPUniversidad Científica del Sur · PE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionResponses of oral-microflora-exposed dental pulp to a triple antibiotic paste (TAP), a mixture of ciprofloxacin, metronidazole, and minocycline in ointment with macrogol and propylene glycol, remain to be fully clarified at the cellular level. This study aimed to elucidate responses of oral-microflora-exposed dental pulp to capping with TAP in mouse molars.

methodsA cavity was prepared on the first molars of 6-week-old mice to expose the dental pulp for 24 h. The exposed pulp was capped with TAP (TAP group) or calcium hydroxide cement (CH group), in addition to the combination of macrogol (M) and propylene glycol (P) (MP, control group), followed by a glass ionomer cement filling. The samples were collected at intervals of 1, 2, and 3 weeks, and immunohistochemistry for nestin and Ki-67 and deoxyuride-5'-triphosphate biotin nick end labeling (TUNEL) assay were performed in addition to quantitative real-time polymerase chain reaction (qRT-PCR) analyses.

resultsThe highest occurrence rate of pulp necrosis was found in the control group followed by the CH group at Weeks 2 and 3, whereas the highest occurrence rate of healed areas in the dental pulp was observed in the TAP group at each time point. Tertiary dentin formation was first observed in the dental pulp of the TAP group at Week 2. In contrast, bone-like and/or fibrous tissues were frequently observed in the CH group. qRT-PCR analyses clarified that TAP activated the stem and dendritic cells at Weeks 1 and 2, respectively.

conclusionsThe use of TAP as a pulp-capping agent improved the healing process of oral-microflora-exposed dental pulp in mouse molars.

Indexed as

ANOVA, One-way analysis of varianceAnti-bacterial agentsAZAN, Azocarmine and aniline blueBirc5, Baculoviral IAP Repeat Containing 5BMPs, Bone morphogenetic proteinscDNA, Complementary deoxyribonucleic acidCell differentiationCell proliferationCH, Calcium hydroxideCt, Cycle thresholdDAP, Double antibiotic pasteDCs, Dendritic cellsDental cavity preparationDental pulpDNA, Deoxyribonucleic acidDPC, Direct pulp cappingDPSCs, Dental pulp stem cellsFGFs, Fibroblast growth factorsGM-CSF, Granulocyte-macrophage colony-stimulating factorH2O2, Hydrogen peroxideHE, Hematoxylin-eosinHLA-DR-immunopositive cells, Human Leukocyte Antigen – DR isotype-immunopositive cellsMHC, Major histocompatibility complexMice (crlj:CD1)M, MacrogolMP, Macrogol (M) mixed with propylene glycol (P)mRNA, Messenger ribonucleic acidMSCs, Mesenchymal stem cellsMTA, Mineral trioxide aggregatemTAP, Modified triple antibiotic pasteOct 3/4 A, Octamer binding transcription factor 3/4 AOct 3/4 B, Octamer binding transcription factor 3/4 BPBS, Phosphate-buffered salinePcna, Proliferating cell nuclear antigenP, Propylene glycolqRT-PCR, Quantitative real-time polymerase chain reactionREP, Regenerative endodontic proceduresRNA, Ribonucleic acidRT, Reverse transcriptionSCAP, Stem cells of the apical papillaSox 10, SRY-related HMG-box 10TAC-P, Triple antibiotic combination and propylene glycolTAC, Triple antibiotic combination (a mixture of metronidazole, ciprofloxacin, and minocycline)TAP, Triple antibiotic pasteTAS, Triple antibiotic solutionTGFβ, Transforming growth factor βTris–HCl buffer, Tris (hydroxymethyl) aminomethane (THAM) hydrochloride bufferTUNEL assay, Terminal deoxynucleotidyl transferase dUTP nick end labeling assayYap1, Yes-associated protein 1αTCP, Alpha tricalcium phosphateβ-actin, Beta-actin

Identifiers

PMID33426222
PMCPMC7770410
OpenAlexW3096170519

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.