ArticleInternational journal of veterinary science and medicine2020
Concurrent detection of bovine viral diarrhoea virus and bovine herpesvirus-1 in bulls' semen and their effect on semen quality.
Article in International journal of veterinary science and medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 19 citations in OpenAlex.
- Research progress on the role and mechanism of DNA damage repair in germ cell development.Frontiers in endocrinology · 2023Pooled it
- Do foot and mouth disease vaccines affect bovine bull fertility? A systematic review and meta-analysis.Veterinary and animal science · 2026Review
- Effects of bovine herpesvirus 1 on thawed bull sperm and evaluation of methods for its removal from experimentally infected semen.Tropical animal health and production · 2026Article
- Antiviral activity of selected plant-derived polyphenols against Varicellovirus bovinealpha1 (BoAHV-1).AMB Express · 2025Article
- Development of a Multiplex Real-Time PCR Assay for the Detection of Eight Pathogens Associated with Bovine Respiratory Disease Complex from Clinical Samples.Microorganisms · 2025Article
- Review
- Development and application of reverse transcriptase droplet digital PCR technology for sensitive detection of BVDV-1 and BTV in bovine semen.BMC veterinary research · 2025Article
- Isolation, characterization and virulence assessment of Type 1d CP bovine viral diarrhea virus originating from Inner Mongolia, China.Frontiers in veterinary science · 2025Article
- Infectious bovine rhinotracheitis: Unveiling the hidden threat to livestock productivity and global trade.Open veterinary journal · 2024Review
- Exploring viral diversity and metagenomics in livestock: insights into disease emergence and spillover risks in cattle.Veterinary research communications · 2024Review
- Prevalence ofVeterinary world · 2024Article
- Exploration of semen quality analyzed by casa-mot systems of brahman bulls infected with BLV and BHV-1.Scientific reports · 2023Article
- What about the bull? A systematic review about the role of males in bovine infectious infertility within cattle herds.Veterinary and animal science · 2023Review
- Non-structural proteins of bovine viral diarrhea virus.Virus genes · 2022Review
- Development of a droplet digital PCR assay to detect bovine alphaherpesvirus 1 in bovine semen.BMC veterinary research · 2022Article
- Impact of Overuse and Sexually Transmitted Infections on Seminal Parameters of Extensively Managed Bulls.Animals : an open access journal from MDPI · 2021Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reproductive diseases may have destructive effects on the fertility of cattle. Bovine viral diarrhoea virus (BVDV) and bovine herpes virus-1 (BoHV-1) are potent viral pathogens linked to reproduction. Thus, the aim of this study was to utilize raw semen samples for conventional and molecular detection of BVDV and BoHV-1, simultaneously. Additionally, the effect of virus infection on the semen quality of naturally infected bulls has been investigated. Therefore, 40 bulls were employed for semen collection, evaluation and testing for both viruses by virus isolation, direct fluorescent antibody technique (FAT) and SYBR Green real-time PCR assay. In virus isolation results, no cytopathic effect (CPE) was observed for BVDV on cell culture whereas, eight (20%) samples displayed characteristic grape-like clusters of cells for BoHV-1. By direct FAT, 12 (30%) positive BVDV and 8 (20%) positive BoHV-1 samples were confirmed. SYBR Green real-time PCR analysis using 48 h inoculated semen samples revealed 14 (35%) and 8 (20%) positive samples for BVDV and BoHV-1, respectively. Statistical analysis of semen evaluation parameters showed a significant difference between viral-infected and free groups represented by increased sperm abnormalities and decreased sperm motility, liveability and concentration. However, there was no significant difference among BVDV, BoHV-1 and mixed-infected groups. The study concluded that BVDV and/or BoHV- 1 infected bulls expressed low semen quality. Real-time PCR was confirmed to be the ideal laboratory assay for detection of both viruses in semen.
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