Evidence map›Paper›PMID 33425987›Full record

ReviewFrontiers in molecular biosciences2020

Next-Generation Molecular Diagnostics Development by CRISPR/Cas Tool: Rapid Detection and Surveillance of Viral Disease Outbreaks.

Sonal Srivastava, Dilip J Upadhyay, Ashish Srivastava

Abstract readReview
In one paragraph

Review in Frontiers in molecular biosciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Review
  5. CRISPR-Cas12 Application for the Detection ofInternational journal of molecular sciences · 2025
    Article
  6. Review
  7. Review
  8. CRISPR-Cas-Based Diagnosis of Geminiviruses.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
  9. Review
  10. Article
  11. Article
  12. Review
  13. Article
  14. HIV infection detection using CRISPR/Cas systems: Present and future prospects.Computational and structural biotechnology journal · 2023
    Review
  15. Review
  16. Review
  17. Review
  18. CRISPR Approaches for the Diagnosis of Human Diseases.International journal of molecular sciences · 2022
    Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Sonal SrivastavaAmity Institute of Virology and Immunology, Amity University Uttar Pradesh, Noida, India.
Dilip J UpadhyayAmity University Uttar Pradesh, Noida, India.
Ashish SrivastavaAmity Institute of Virology and Immunology, Amity University Uttar Pradesh, Noida, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Virus disease spreads effortlessly mechanically or through minute insect vectors that are extremely challenging to avoid. Emergence and reemergence of new viruses such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), H1N1 influenza virus, avian influenza virus, dengue virus, Citrus tristeza virus, and Tomato yellow leaf curl virus have paralyzed the economy of many countries. The cure for major viral diseases is not feasible; however, early detection and surveillance of the disease can obstruct their spread. Therefore, advances in the field of virus diagnosis and the development of new point-of-care testing kits become necessary globally. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) is an emerging technology for gene editing and diagnostics development. Several rapid nucleic acid diagnostic kits have been developed and validated using Cas9, Cas12, and Cas13 proteins. This review summarizes the CRISPR/Cas-based next-generation molecular diagnostic techniques and portability of devices for field-based utilization.

Indexed as

Cas12Cas13Cas9CRISPR associated proteinsisothermal amplificationNAT

Identifiers

PMID33425987
PMCPMC7785713

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.