ArticleFrontiers in microbiology2020
Development of a Directly Visualized Recombinase Polymerase Amplification-SYBR Green I Method for the Rapid Detection of African Swine Fever Virus.
Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Selenoprotein S Deficiency Induces Matrix Degradation Via TGF-β Pathway Leading to Cartilage Damage.Biological trace element research · 2026Article
- Recent advances in isothermal amplification techniques for detection of animal diseases.Frontiers in veterinary science · 2026Review
- Review
- Rapid visual detection of bovine viral diarrhea virus (BVDV) using recombinase polymerase amplification with SYBR green I.BMC veterinary research · 2025Article
- Development of isothermal nucleic acid amplification technologies for rapid detection of Porcine Enterovirus-G.PloS one · 2025Article
- Strategic nucleic acid detection approaches for diagnosing African swine fever (ASF): navigating disease dynamics.Veterinary research · 2024Review
- Simultaneous detection of bovine viral diarrhea virus (BVDV) and bovine herpesvirus 1 (BoHV-1) using recombinase polymerase amplification.Scientific reports · 2024Article
- Development of a novel sensitive single-tube nested PCR assay for the detection of African swine fever virus.Archives of virology · 2024Article
- Quickly assessing disinfection effectiveness to control the spread of African swine fever virus.Applied microbiology and biotechnology · 2023Article
- Novel sensitive isothermal-based diagnostic technique for the detection of African swine fever virus.Archives of virology · 2023Article
- Application of propidium monoazide quantitative PCR to discriminate of infectious African swine fever viruses.Frontiers in microbiology · 2023Article
- Technical Evaluation of qPCR Multiplex Assays for the Detection ofMicroorganisms · 2022Article
- Non-nucleic acid extraction and ultra-sensitive detection of African swine fever virus via CRISPR/Cas12a.Applied microbiology and biotechnology · 2022Article
- Development of a Real-Time Recombinase Polymerase Amplification Assay for the Rapid Detection of African Swine Fever Virus Genotype I and II.Pathogens (Basel, Switzerland) · 2022Article
- Evaluation of a Lateral Flow Assay for Rapid Detection of African Swine Fever Virus in Multiple Sample Types.Pathogens (Basel, Switzerland) · 2022Article
- Development and application of a colloidal-gold dual immunochromatography strip for detecting African swine fever virus antibodies.Applied microbiology and biotechnology · 2022Article
- Rapid visual detection of anisakid nematodes using recombinase polymerase amplification and SYBR Green I.Frontiers in microbiology · 2022Article
- Prospects for the application of infectious virus detection technology based on propidium monoazide in African swine fever management.Frontiers in microbiology · 2022Review
- A Novel, Cleaved Probe-Based Reverse Transcription Loop-Mediated Isothermal Amplification Method for Specific and Sensitive Detection of Porcine Deltacoronavirus.Frontiers in veterinary science · 2022Article
- The Development of a Real-Time Recombinase-Aid Amplification Assay for Rapid Detection of African Swine Fever Virus.Frontiers in microbiology · 2022Article
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11 authors.
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No grant is acknowledged in the PubMed record.
Abstract
African swine fever (ASF) is a lethal disease in swine caused by etiologic African swine fever virus (ASFV). The global spread of ASFV has resulted in huge economic losses globally. In the absence of effective vaccines or drugs, pathogen surveillance has been the most important first-line intervention to prevent ASF outbreaks. Among numerous diagnostic methods, recombinase polymerase amplification (RPA)-based detection is capable of producing sensitive and specific results without relying on the use of expensive instruments. However, currently used gene-specific, probe-based RPA for ASFV detection is expensive and time-consuming. To improve the efficiency of ASFV surveillance, a novel directly visualized SYBR Green I-staining RPA (RPAS) method was developed to detect the ASFV genome. SYBR Green I was added to the amplified RPA products for direct visualization by the naked eye. The sensitivity and specificity of this method were confirmed using standard plasmid and inactivated field samples. This method was shown to be highly specific with a detection limit of 10
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