ArticleThe New phytologist2021
Growth-regulating factor 5 (GRF5)-mediated gene regulatory network promotes leaf growth and expansion in poplar.
Article in The New phytologist, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers, 1 of them a synthesis that pooled it.
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Who cites it
43 citing papers in PubMed, 1 synthesis or guideline pooled it, 83 citations in OpenAlex.
- Overview of molecular mechanisms of plant leaf development: a systematic review.Frontiers in plant science · 2023Pooled it
- An Intronic Structural Variant in GhEBS1 Drives Cotton Fruit Size Divergence by Modulating Chromatin Accessibility and Gene Expression.Plant biotechnology journal · 2026Article
- Article
- The E3 ubiquitin ligase RHB1A and the transcription factor GRF1 form a regulatory module that orchestrates the trade-off between photosynthetic growth and salt tolerance in poplar.Plant communications · 2026Article
- PtCP1 Is an Extraplastidial Cysteine Protease Involved in Leaf Protein Degradation ofPlants (Basel, Switzerland) · 2026Article
- The GATA8-GRF5-XTH9 feed-forward loop regulates cell size in poplar.Horticulture research · 2026Article
- Genome-wide identification of the GRF gene family and response to cold stress in astragalus (Astragalus membranaceus).BMC genomics · 2026Article
- Genome-Wide Identification and Expression Analysis of theInternational journal of molecular sciences · 2026Article
- Autopolyploidization-Induced Chromatin Remodeling Regulates Leaf Size Variation in Brassica rapa.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A comparative study of phyllostachys edulis and its dwarf variant phyllostachys edulis 'Tubaeformis' at the anatomical, transcriptomic, and DNA methylation levels.Frontiers in plant science · 2026Article
- Rootstock of distant hybrid activates transcription factors of scion cultivar to promote growth and yield in walnut.Plant biotechnology journal · 2025Article
- Genome-wide characterization and expression analysis of the growth-regulating factor family in Mikania micrantha.BMC plant biology · 2025Article
- Hormonal and Transcriptomic Insights into Inflorescence Stalk Elongation in Oil Palm.Plants (Basel, Switzerland) · 2025Article
- GWAS-based population genetic analysis identifies bZIP29 as a heterotic gene in maize.Plant communications · 2025Article
- Genome-Wide Identification and Characterization of the Growth-Regulating Factor Gene Family Responsive to Abiotic Stresses and Phytohormone Treatments inInternational journal of molecular sciences · 2025Article
- Increased genomic predictive ability in mango using GWAS-preselected variants and fixed-effect SNPs.Frontiers in plant science · 2025Article
- Genome-wide identification of growth-regulating factor andFrontiers in plant science · 2025Article
- Overexpression of Vitis GRF4-GIF1 improves regeneration efficiency in diploid Fragaria vesca Hawaii 4.Plant methods · 2024Article
- Functional Mechanisms and the Application of Developmental Regulators for Improving Genetic Transformation in Plants.Plants (Basel, Switzerland) · 2024Review
- Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although polyploid plants have larger leaves than their diploid counterparts, the molecular mechanisms underlying this difference (or trait) remain elusive. Differentially expressed genes (DEGs) between triploid and full-sib diploid poplar trees were identified from two transcriptomic data sets followed by a gene association study among DEGs to identify key leaf growth regulators. Yeast one-hybrid system, electrophoretic mobility shift assay, and dual-luciferase assay were employed to substantiate that PpnGRF5-1 directly regulated PpnCKX1. The interactions between PpnGRF5-1 and growth-regulating factor (GRF)-interacting factors (GIFs) were experimentally validated and a multilayered hierarchical regulatory network (ML-hGRN)-mediated by PpnGRF5-1 was constructed with top-down graphic Gaussian model (GGM) algorithm by combining RNA-sequencing data from its overexpression lines and DAP-sequencing data. PpnGRF5-1 is a negative regulator of PpnCKX1. Overexpression of PpnGRF5-1 in diploid transgenic lines resulted in larger leaves resembling those of triploids, and significantly increased zeatin and isopentenyladenine in the apical buds and third leaves. PpnGRF5-1 also interacted with GIFs to increase its regulatory diversity and capacity. An ML-hGRN-mediated by PpnGRF5-1 was obtained and could largely elucidate larger leaves. PpnGRF5-1 and the ML-hGRN-mediated by PpnGRF5-1 were underlying the leaf growth and development.
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