Evidence map›Paper›PMID 33423224›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Skin Equivalent Models: Protocols for In Vitro Reconstruction for Dermal Toxicity Evaluation.

Tatiana do Nascimento Pedrosa, Carolina Motter Catarino, Paula Comune Pennacchi, Silvia Berlanga de Moraes Barros, Silvya Stuchi Maria-Engler

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
9.2field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. AlternativeExpert reviews in molecular medicine · 2026
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Tatiana do Nascimento PedrosaSkin Biology and Melanoma Laboratory, School of Pharmaceutical Sciences, University of São Paulo, Butantã, SP, Brazil.
Carolina Motter CatarinoSkin Biology and Melanoma Laboratory, School of Pharmaceutical Sciences, University of São Paulo, Butantã, SP, Brazil.
Paula Comune PennacchiSkin Biology and Melanoma Laboratory, School of Pharmaceutical Sciences, University of São Paulo, Butantã, SP, Brazil.
Silvia Berlanga de Moraes BarrosSkin Biology and Melanoma Laboratory, School of Pharmaceutical Sciences, University of São Paulo, Butantã, SP, Brazil.
Silvya Stuchi Maria-EnglerSkin Biology and Melanoma Laboratory, School of Pharmaceutical Sciences, University of São Paulo, Butantã, SP, Brazil. silvya@usp.br.
Instituto Butantan · BRUniversidade de São Paulo · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter presents the protocols for developing of skin equivalents (SE) and reconstructed human epidermis (RHE) models for dermal toxicity evaluation as an alternative method to animal use in research. It provides a detailed protocol for the in vitro reconstruction of human skin from primary keratinocytes, melanocytes, and fibroblasts obtained from foreskin biopsies, including the procedures for reconstruction of a stratified epidermis on a polyester membrane. SE and RHE developed through these methods have been proven suitable not only for dermal toxicity studies, but also for investigating of pathological conditions in the skin, such as diabetes and invasion of melanoma.

Indexed as

Cells, CulturedEpidermisHumansPrimary Cell CultureSkin Irritancy TestsHuman primary cellsPrimary cultureReconstructed human epidermis (RHE)Skin equivalent (SE)

Identifiers

PMID33423224
OpenAlexW3121071086

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.