ArticleNucleic acids research2021
A supernumerary designer chromosome for modular in vivo pathway assembly in Saccharomyces cerevisiae.
Article in Nucleic acids research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Advances in large DNA fragment assembly for microbial cell factory engineering.Quantitative biology (Beijing, China) · 2026Review
- One Yeast, Sixteen Synthetic Chromosomes, Infinite Possibilities.Yeast (Chichester, England) · 2026Review
- PYEAST - A Computational Toolkit for Saccharomyces cerevisiae Genetic Engineering.NPJ systems biology and applications · 2026Article
- Article
- Intergeneric chromosomal transfer in yeast results in improved phenotypes and widespread transcriptional responses.Nature communications · 2026Article
- Life beyond 6000 genes: from sequence to synthesis in a post-genomics era.FEMS yeast research · 2026Review
- The design and engineering of synthetic genomes.Nature reviews. Genetics · 2025Review
- Krisp: A Python package to aid in the design of CRISPR and amplification-based diagnostic assays from whole genome sequencing data.PLoS computational biology · 2024Article
- Convenient synthesis and delivery of a megabase-scale designer accessory chromosome empower biosynthetic capacity.Cell research · 2024Article
- Article
- A supernumerary synthetic chromosome in Komagataella phaffii as a repository for extraneous genetic material.Microbial cell factories · 2023Article
- Application and Technical Challenges in Design, Cloning, and Transfer of Large DNA.Bioengineering (Basel, Switzerland) · 2023Review
- Expanding the genome editing toolbox of Saccharomyces cerevisiae with the endonuclease ErCas12a.FEMS yeast research · 2023Article
- Challenges to Ensure a Better Translation of Metabolic Engineering for Industrial Applications.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Construction and evaluation of gRNA arrays for multiplex CRISPR-Cas9.Yeast (Chichester, England) · 2023Article
- Article
- Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design.ACS synthetic biology · 2022Article
- Exogenous artificial DNA forms chromatin structure with active transcription in yeast.Science China. Life sciences · 2022Article
- Synthetic Genomics From a Yeast Perspective.Frontiers in bioengineering and biotechnology · 2022Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The construction of microbial cell factories for sustainable production of chemicals and pharmaceuticals requires extensive genome engineering. Using Saccharomyces cerevisiae, this study proposes synthetic neochromosomes as orthogonal expression platforms for rewiring native cellular processes and implementing new functionalities. Capitalizing the powerful homologous recombination capability of S. cerevisiae, modular neochromosomes of 50 and 100 kb were fully assembled de novo from up to 44 transcriptional-unit-sized fragments in a single transformation. These assemblies were remarkably efficient and faithful to their in silico design. Neochromosomes made of non-coding DNA were stably replicated and segregated irrespective of their size without affecting the physiology of their host. These non-coding neochromosomes were successfully used as landing pad and as exclusive expression platform for the essential glycolytic pathway. This work pushes the limit of DNA assembly in S. cerevisiae and paves the way for de novo designer chromosomes as modular genome engineering platforms in S. cerevisiae.
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Registered trials
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