ArticlePlant methods2021
Split-root systems: detailed methodology, alternative applications, and implications at leaf proteome level.
Article in Plant methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 26 citations in OpenAlex.
- Establishment of a rapid split-root assay in hydroponic conditions for eight upland cotton varieties.MethodsX · 2025Article
- Translating soil salinity to agricultural salt stress: Key salt-tolerance mechanisms for agrohydrologic models.iScience · 2025Review
- CORONATINE INSENSITIVE 1-mediated repression of immunity-related genes in Arabidopsis roots is lifted upon infection with Verticillium longisporum.Journal of experimental botany · 2025Article
- Systemic signals control infection plasticity in parasitic plants.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Effectiveness of Plant-Induced Resistance Against Root-Knot Nematode Depends on the Policy of Using Inducer on the Host Plant.Current microbiology · 2025Article
- Ensuring robustness in scientific research, split-root assays as an example case.Quantitative plant biology · 2025Article
- Neighbour-induced changes in root exudation patterns of buckwheat results in altered root architecture of redroot pigweed.Scientific reports · 2024Article
- Competition for light induces metal accumulation in a metal hyperaccumulating plant.Oecologia · 2021Article
- Specialized Plant Growth Chamber Designs to Study Complex Rhizosphere Interactions.Frontiers in microbiology · 2021Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundSplit-root systems (SRS) have many applications in plant sciences, but their implementation, depending on the experimental design, can be difficult and time-consuming. Additionally, the system is not exempt from limitations, since the time required for the establishment of the SRS imposes a limit to how early in plant development experiments can be performed. Here, we optimized and explained in detail a method for establishing a SRS in young Arabidopsis thaliana seedlings, both in vitro and in soil.
resultsWe found that the partial de-rooting minimized the recovery time compared to total de-rooting, thus allowing the establishment of the split-root system in younger plants. Analysis of changes in the Arabidopsis leaf proteome following the de-rooting procedure highlighted the distinct metabolic alterations that totally and partially de-rooted plants undergo during the healing process. This system was also validated for its use in drought experiments, as it offers a way to apply water-soluble compounds to plants subjected to drought stress. By growing plants in a split-root system with both halves being water-deprived, it is possible to apply the required compound to one half of the root system, which can be cut from the main plant once the compound has been absorbed, thus minimizing rehydration and maintaining drought conditions.
conclusionsPartial de-rooting is the suggested method for obtaining split-root systems in small plants like Arabidopsis thaliana, as growth parameters, survival rate, and proteomic analysis suggest that is a less stressful procedure than total de-rooting, leading to a final rosette area much closer to that of uncut plants. Additionally, we provide evidence that split root-systems can be used in drought experiments where water-soluble compounds are applied with minimal effects of rehydration.
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