ArticleMalaria journal2021
New assessment of Anopheles vector species identification using MALDI-TOF MS.
Article in Malaria journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 46 citations in OpenAlex.
- Intraspecific geographical variability of Phlebotomus perniciosus assessed by MALDI-TOF MS protein profiling.Parasites & vectors · 2026Article
- Glossina from the Republic of the Congo: species identification by MALDI-TOF MS and research of associated micro-organisms.Parasite (Paris, France) · 2026Article
- MALDI-TOF mass spectrometry combined with machine learning algorithms to identify protein profiles related to malaria infection in human sera from Côte d'Ivoire.Malaria journal · 2025Article
- Assessment of MALDI-TOF MS for Arthropod Identification Based on Exuviae Spectra Analysis.Biological procedures online · 2025Article
- Identification of Southeast Asian Anopheles mosquito species with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using a cross-correlation approach.Parasites & vectors · 2025Article
- Efficiency of MALDI-TOF MS at identifying and discriminating immature stages of cimex lectularius and cimex hemipterus bed bugs.Scientific reports · 2024Article
- MALDI-TOF MS Profiling and Its Contribution to Mosquito-Borne Diseases: A Systematic Review.Insects · 2024Review
- Predicting the age of fieldScience advances · 2024Article
- A universal tool for marine metazoan species identification: towards best practices in proteomic fingerprinting.Scientific reports · 2024Article
- Identification of Southeast Asian Anopheles mosquito species using MALDI-TOF mass spectrometry.PloS one · 2024Article
- Deep learning and wing interferential patterns identify Anopheles species and discriminate amongst Gambiae complex species.Scientific reports · 2023Article
- Identification of NeotropicalTropical medicine and infectious disease · 2023Article
- Article
- Utility of MALDI-TOF MS for determination of species identity and blood meal sources of primary malaria vectors on the Kenyan coast.Wellcome open research · 2023Article
- High-resolution species assignment ofeLife · 2022Article
- Enhanced procedures for mosquito identification by MALDI-TOF MS.Parasites & vectors · 2022Article
- MALDI-TOF mass spectrometry identification of mosquitoes collected in Vietnam.Parasites & vectors · 2022Article
- Article
Corrections and comments
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Authors and funding
14 authors at 7 institutions in 7 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAnopheles species identification is essential for an effective malaria vector control programme. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has been developed to identify adult Anopheles species, using the legs or the cephalothorax. The protein repertoire from arthropods can vary according to compartment, but there is no general consensus regarding the anatomic part to be used.
methodsTo determine the body part of the Anopheles mosquitoes best suited for the identification of field specimens, a mass spectral library was generated with head, thorax with wings and legs of Anopheles gambiae, Anopheles arabiensis and Anopheles funestus obtained from reference centres. The MSL was evaluated using two independent panels of 52 and 40 An. gambiae field-collected in Mali and Guinea, respectively. Geographic variability was also tested using the panel from Mali and several databases containing added specimens from Mali and Senegal.
resultsUsing the head and a database without specimens from the same field collection, the proportion of interpretable and correct identifications was significantly higher than using the other body parts at a threshold value of 1.7 (p < 0.0001). The thorax of engorged specimens was negatively impacted by the blood meal after frozen storage. The addition of specimens from Mali into the database significantly improved the results of Mali panel (p < 0.0001), which became comparable between head and legs. With higher identification scores, the using of the head will allow to decrease the number of technical replicates of protein extract per specimen, which represents a significant improvement for routine use of MALDI-TOF MS.
conclusionsThe using of the head of Anopheles may improve the performance of MALDI-TOF MS. Region-specific mass spectrum databases will have to be produced. Further research is needed to improve the standardization in order to share online spectral databases.
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