ArticleJournal of mass spectrometry : JMS2021
Evaluation of three sample preparation methods for the identification of clinical strains by using two MALDI-TOF MS systems.
Article in Journal of mass spectrometry : JMS, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 41 citations in OpenAlex.
- Enhanced classification and identification of bacterial and viral microorganisms by integration of MALDI-TOF mass spectrometry with artificial intelligence.Scientific reports · 2026Article
- Genomic Characterization and Virulence Determinants ofAntibiotics (Basel, Switzerland) · 2026Article
- Rapid Bacterial Identification and Quantitative Antimicrobial Susceptibility Assessment from Positive Blood Cultures to Optimize Bloodstream Infection Management.Microorganisms · 2026Article
- A Pilot Study ofDiagnostics (Basel, Switzerland) · 2026Article
- Diversity of grass-associated Nitrospirillum and proposal of six novel species.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- MALDI-TOF MS Biomarkers for Methicillin-ResistantMetabolites · 2025Review
- Spectral Precision: Recent Advances in Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for Pathogen Detection and Resistance Profiling.Microorganisms · 2025Review
- Identification of Challenging Dermatophyte Species Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.Journal of fungi (Basel, Switzerland) · 2025Article
- Identification of Legionella by MALDI Biotyper through three preparation methods and an in-house library comparing phylogenetic and hierarchical cluster results.Scientific reports · 2025Article
- Diagnostic performance of an automated robot for MALDI target preparation in microbial identification.Journal of clinical microbiology · 2024Article
- Clinical Evaluation of VITEK MS PRIME with PICKME Pen for Bacteria and Yeasts, and RUO Database for Filamentous Fungi.Microorganisms · 2024Article
- Nitrospirillum viridazoti sp. nov., an Efficient Nitrogen-Fixing Species Isolated from Grasses.Current microbiology · 2024Article
- Identification of environmental Actinobacteria in buildings by means of chemotaxonomy, 16S rRNA sequencing, and MALDI-TOF MS.Microbiology spectrum · 2024Article
- MALDI-TOF MS: A Reliable Tool in the Real Life of the Clinical Microbiology Laboratory.Microorganisms · 2024Review
- A universal tool for marine metazoan species identification: towards best practices in proteomic fingerprinting.Scientific reports · 2024Article
- Effects of Embryo Microbial Contamination on ART and Neonatal Outcomes.Infection and drug resistance · 2024Article
- Detection ofFrontiers in cellular and infection microbiology · 2024Article
- Performance assessment of the Bruker Biotyper MALDI-TOF MS for the identification of difficult-to-identify viridans group streptococci.Journal of clinical microbiology · 2023Article
- Article
- Evaluation of the Zybio EXS3000 mass spectrometry in routine identification of Clinical isolates.Heliyon · 2023Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has revolutionized the microbial identification, especially in the clinical microbiology laboratories. However, although numerous studies on the identification of microorganisms by MALDI-TOF MS have been reported previously, few studies focused on the effect of pretreatment on identification. Due to the sensitivity of MALDI-TOF MS, different preparation methods will lead to changes in microbial protein fingerprints. In this study, for evaluating a more appropriate preparation method for the clinical microbiology identification, we analyzed the performance of three sample preparation methods on two different MALDI-TOF MS systems. A total of 321 clinical isolates, 127 species, were employed in the comparative study of three different sample preparation methods including the direct colony transfer method (DCTM), the on-target extraction method (OTEM), and the in-tube extraction method (ITEM) compatible with MALDI-TOF MS. All isolates were tested on the Microflex LT and Autof ms1000 devices. The spectra were analyzed using the Bruker biotyper and the Autof ms1000 systems. The results were confirmed by 16/18S rRNA sequencing. Results reveal that the accuracies of isolates identification by Bruker biotyper successfully identified 83.8%, 96.0%, and 95.3% after performing the DCTM, OTEM, and ITEM, respectively, while the Autof ms1000 identified 97.5%, 100%, and 99.7%. These data suggested that the identification rates are comparable among the three preparation methods using the Autof ms1000 and Bruker microflex LT systems but the OTEM is more suitable and necessary for clinical application, owing to its key advantages of simplicity and accuracy.
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