ArticleThe New phytologist2021
A blueprint for gene function analysis through Base Editing in the model plant Physcomitrium (Physcomitrella) patens.
Article in The New phytologist, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Functional divergence of LhcSR and PsbS in zeaxanthin-mediated non-photochemical quenching.The New phytologist · 2026Article
- tRNA-based polycistronic CRISPR/Cas9 system boosts efficiency of multi-gene deletion in the moss Physcomitrella.Plant methods · 2026Article
- The SpRY Cas9 variant release the PAM sequence constraint for genome editing in the model plant Physcomitrium patens.Transgenic research · 2024Article
- Improved prime editing allows for routine predictable gene editing in Physcomitrium patens.Journal of experimental botany · 2023Article
- Blueprint for non-transgenic edited plants.Nature plants · 2023Article
- What can hornworts teach us?Frontiers in plant science · 2023Review
- Biotechnological Advances to Improve Abiotic Stress Tolerance in Crops.International journal of molecular sciences · 2022Review
- Genome-wide specificity of plant genome editing by both CRISPR-Cas9 and TALEN.Scientific reports · 2022Article
- Homology-based identification of candidate genes for male sterility editing in upland cotton (Frontiers in plant science · 2022Review
- Genome editing techniques in plants: a comprehensive review and future prospects toward zero hunger.GM crops & food · 2021Review
- Precise plant genome editing using base editors and prime editors.Nature plants · 2021Review
- Targeted Gene Knockouts by Protoplast Transformation in the MossFrontiers in genome editing · 2021Review
- Protoplast: A Valuable Toolbox to Investigate Plant Stress Perception and Response.Frontiers in plant science · 2021Review
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRISPR-Cas9 has proven to be highly valuable for genome editing in plants, including the model plant Physcomitrium patens. However, the fact that most of the editing events produced using the native Cas9 nuclease correspond to small insertions and deletions is a limitation. CRISPR-Cas9 base editors enable targeted mutation of single nucleotides in eukaryotic genomes and therefore overcome this limitation. Here, we report two programmable base-editing systems to induce precise cytosine or adenine conversions in P. patens. Using cytosine or adenine base editors, site-specific single-base mutations can be achieved with an efficiency up to 55%, without off-target mutations. Using the APT gene as a reporter of editing, we could show that both base editors can be used in simplex or multiplex, allowing for the production of protein variants with multiple amino-acid changes. Finally, we set up a co-editing selection system, named selecting modification of APRT to report gene targeting (SMART), allowing up to 90% efficiency site-specific base editing in P. patens. These two base editors will facilitate gene functional analysis in P. patens, allowing for site-specific editing of a given base through single sgRNA base editing or for in planta evolution of a given gene through the production of randomly mutagenised variants using multiple sgRNA base editing.
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