Evidence map›Paper›PMID 33420999›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Chromatin Immunoprecipitation (ChIP) to Study DNA-Protein Interactions.

Eliza C Small, Danielle N Maryanski, Keli L Rodriguez, Kevin J Harvey, Michael-C Keogh, Andrea L Johnstone

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. High-Efficiency Genomic Mapping of Chromatin-Associated Targets with CUT&RUN.Methods in molecular biology (Clifton, N.J.) · 2026
    Article
  2. Review
  3. Review
  4. Article
  5. Article
  6. ChIP-Seq Analysis ofFoods (Basel, Switzerland) · 2023
    Article
  7. ChIP and ChIRP Assays in Ferroptosis.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
  8. Article
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Eliza C SmallThermo Fisher Scientific, Rockford, IL, USA. eliza.small@thermofisher.com.
Danielle N MaryanskiEpiCypher Inc., Durham, NC, USA.
Keli L RodriguezEpiCypher Inc., Durham, NC, USA.
Kevin J HarveyThermo Fisher Scientific, Carlsbad, CA, USA.
Michael-C KeoghEpiCypher Inc., Durham, NC, USA.
Andrea L JohnstoneEpiCypher Inc., Durham, NC, USA.

Funding

Internally Calibrated Chromatin Immunoprecipitation Using Barcoded NucleosomesR44HG008907 · NHGRI · EPICYPHER, INC. · PI KEOGH, MICHAEL-CHRISTOPHER · 2016 to 2017
$1.6M
Barcoded nucleosomes for analyzing combinatorial epigenetic regulatorsR44GM116584 · NIGMS · EPICYPHER, INC. · PI SUN, ZU-WEN · 2017 to 2018
$1.6M
Quantitative assessment of response to epigenetic-targeted therapyR43CA232941 · NCI · EPICYPHER, INC. · PI KEOGH, MICHAEL-CHRISTOPHER · 2019 to 2019
$300k
NCI NIH HHS R43 CA232941NHGRI NIH HHS R44 HG008907NIGMS NIH HHS R44 GM116584
6 · The paper itself

Abstract

Chromatin immunoprecipitation (ChIP) is a method used to examine the genomic localization of a target of interest (e.g., proteins, protein posttranslational modifications, or DNA elements). As ChIP provides a snapshot of in vivo DNA-protein interactions, it lends insight to the mechanisms of gene expression and genome regulation. This chapter provides a detailed protocol focused on native-ChIP (N-ChIP), a robust approach to profile stable DNA-protein interactions. We also describe best practices for ChIP , including defined controls to ensure specific and efficient target enrichment and methods for data normalization.

Indexed as

Chromatin ImmunoprecipitationAnimalsCells, CulturedChromatinDNAHistonesHumansProtein BindingProtein Processing, Post-TranslationalWorkflowChromatinDNAHistonesAntibody specificityChIPChIP normalizationChromatin immunoprecipitationHistone PTMsRecombinant nucleosomesSemisynthetic nucleosomesSpike-in controls

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.