Evidence map›Paper›PMID 33420994›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Multiplex Fluorescent Bead-Based Immunoassay for the Detection of Cytokines, Chemokines, and Growth Factors.

Jonathan M Preuss, Ute Burret, Sabine Vettorazzi

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.6field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Jonathan M PreussInstitute of Comparative Molecular Endocrinology (CME), Ulm University, Ulm, Germany. jonathan.preuss@uni-ulm.de.
Ute BurretInstitute of Comparative Molecular Endocrinology (CME), Ulm University, Ulm, Germany.
Sabine VettorazziInstitute of Comparative Molecular Endocrinology (CME), Ulm University, Ulm, Germany. sabine.vettorazzi@uni-ulm.de.
Universität Ulm · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The comprehensive analysis of serum cytokine levels can be challenging due to low sample volumes and time consuming when using single-target methods like enzyme-linked immunosorbent assay (ELISA). Bead-based detection systems allow the simultaneous detection of multiple analytes using minimal sample volumes. Here we describe the use of a multiplex cytokine, chemokine, and growth factor assay for mouse cytokines in a 96-well format. This assay is based on antibody-coupled fluorescent magnetic beads combined with biotinylated secondary detection antibody followed by fluorescent-tagged streptavidin in a sandwich-like composition. Final assay readout provides the concentrations of 23 different cytokines, chemokines, and growth factors in up to 76 samples.

Indexed as

Fluorescent Antibody Technique, IndirectFluoroimmunoassayProteomicsAnimalsBiotinylationChemokinesCytokinesDisease Models, AnimalEndotoxemiaIntercellular Signaling Peptides and ProteinsLipopolysaccharidesMiceMice, KnockoutReceptors, GlucocorticoidChemokinesCytokinesIntercellular Signaling Peptides and ProteinsLipopolysaccharidesReceptors, GlucocorticoidBead-basedBioplexChemokineCytokineEndotoxemiaGlucocorticoid receptorInflammationIntensive careMultiplexSerum

Identifiers

PMID33420994
OpenAlexW3119927666

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.