ArticleMethods in molecular biology (Clifton, N.J.)2021
Lab-on-a-Filter Techniques for Economical, Effective, and Flexible Proteome Analysis.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed, 3 citations in OpenAlex.
- Quenching Trypsin Is Unnecessary in Filter-Based Bottom-Up Proteomics.Journal of the American Society for Mass Spectrometry · 2024Article
- Development of an efficient, effective, and economical technology for proteome analysis.Cell reports methods · 2024Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
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Abstract
Effective and reliable protease digestion of biological samples is critical to the success in bottom-up proteomics analysis. Various filter-based approaches using different types of membranes have been developed in the past several years and largely implemented in sample preparations for modern proteomics. However, these approaches rely heavily on commercial filter products, which are not only costly but also limited in membrane options. Here, we present a plug-and-play device for filter assembly and protease digestion. The device can accommodate a variety of membrane types, can be packed in-house with minimal difficulty, and is extremely cost-effective and reliable. Our protocol offers a versatile platform for general proteome analyses and clinical mass spectrometry.
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