ArticleMetabolomics : Official journal of the Metabolomic Society2021
Optimization of metabolite extraction and analytical methods from shrimp intestine for metabolomics profile analysis using LC-HRMS/MS.
Article in Metabolomics : Official journal of the Metabolomic Society, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Investigating a novel β-glucan source to enhance disease resistance in Pacific white shrimp (Penaeus vannamei).Scientific reports · 2025Article
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5 authors.
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Abstract
introductionIntestinal microbiota and metabolites play important roles for further improvement of animal production. Metabolomics of shrimp intestine to understand roles and their relationship to the host is hampered by the lack of metabolome profiling method.
objectivesThis study aims to develop extraction and analytical methods to allow accurate metabolic analysis in shrimp intestine.
methodsConditions for extraction and LC-HRMS/MS analysis were optimized.
resultsExtraction with ethyl acetate:acetone (15:2 v/v) acidified with 0.5% acetic acid, elution with acetonitrile:water acidified with 0.01% acetic acid for 25 min, and mass fragmentation at 15% HCD were the optimal conditions, yielding the highest signal intensity and numbers of putative metabolites.
conclusionOur method enabled in-depth study for shrimp-microbial interaction at metabolite level.
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