ArticleCommunications biology2021
The spatial RNA integrity number assay for in situ evaluation of transcriptome quality.
Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 2 of them syntheses that pooled it.
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Who cites it
19 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Enhancing RNA Capture Efficiency in Spatial Transcriptomics: A Review of Innovative Technologies and Strategies.International journal of molecular sciences · 2025Pooled it
- Potential Role of mRNA in Estimating Postmortem Interval: A Systematic Review.International journal of molecular sciences · 2024Pooled it
- Integrity of Tumor Tissue Collected by Ultrasonic Aspirator: A Cellular and Molecular Perspective.Neurosurgery practice · 2026Article
- RNA-aware tissue preservation workflows for high-quality spatial transcriptomics.bioRxiv : the preprint server for biology · 2026Article
- Animal models in molecular biology research: Challenges, ethical imperatives, and the path to human-relevant translation.Animal models and experimental medicine · 2026Review
- Article
- Enhanced RNA Preservation in Mouse Brain Tissue: A Strategy Combining Cardiac Perfusion with Hypersaline Immersion.ACS omega · 2025Article
- Dual probe ligation in situ hybridization with rolling-circle amplification for high-plex spatial transcriptomics.Biochemistry and biophysics reports · 2025Article
- Spatially resolved genome-wide joint profiling of epigenome and transcriptome with spatial-ATAC-RNA-seq and spatial-CUT&Tag-RNA-seq.Nature protocols · 2025Review
- Decoder-seq enhances mRNA capture efficiency in spatial RNA sequencing.Nature biotechnology · 2024Article
- Spatially resolved epigenome sequencing via Tn5 transposition and deterministic DNA barcoding in tissue.Nature protocols · 2024Review
- Article
- Responses of transcriptome and metabolome in peanut leaves to dibutyl phthalate during whole growth period.Frontiers in plant science · 2024Article
- Microglial contribution to the pathology of neurodevelopmental disorders in humans.Acta neuropathologica · 2023Review
- The emerging landscape of spatial profiling technologies.Nature reviews. Genetics · 2022Review
- Integrated single cell and spatial transcriptomics reveal autoreactive differentiated B cells in joints of early rheumatoid arthritis.Scientific reports · 2022Article
- Opportunities and Challenges in Understanding Atherosclerosis by Human Biospecimen Studies.Frontiers in cardiovascular medicine · 2022Review
- Clinical challenges of tissue preparation for spatial transcriptome.Clinical and translational medicine · 2022Article
- The spatial RNA integrity number assay for in situ evaluation of transcriptome quality.Communications biology · 2021Article
Corrections and comments
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Authors and funding
17 authors.
Funding
Abstract
The RNA integrity number (RIN) is a frequently used quality metric to assess the completeness of rRNA, as a proxy for the corresponding mRNA in a tissue. Current methods operate at bulk resolution and provide a single average estimate for the whole sample. Spatial transcriptomics technologies have emerged and shown their value by placing gene expression into a tissue context, resulting in transcriptional information from all tissue regions. Thus, the ability to estimate RNA quality in situ has become of utmost importance to overcome the limitation with a bulk rRNA measurement. Here we show a new tool, the spatial RNA integrity number (sRIN) assay, to assess the rRNA completeness in a tissue wide manner at cellular resolution. We demonstrate the use of sRIN to identify spatial variation in tissue quality prior to more comprehensive spatial transcriptomics workflows.
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Registered trials
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