ArticleScientific reports2021
Establish axenic cultures of armored and unarmored marine dinoflagellate species using density separation, antibacterial treatments and stepwise dilution selection.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 15 citations in OpenAlex.
- Nitrogen concentration influences growth performance and biochemical composition of the endosymbiotic dinoflagellate Durusdinium Glynnii.Archives of microbiology · 2025Article
- Testing the Purity ofCells · 2025Article
- The need for smart microalgal bioprospecting.Natural products and bioprospecting · 2025Review
- Long-Read Sequencing Unlocks New Insights into theMarine drugs · 2024Article
- Screening of antibiotics to obtain axenic cell cultures of a marine microalgaFrontiers in bioengineering and biotechnology · 2023Article
- Microalgal Biodiesel Production: Realizing the Sustainability Index.Frontiers in bioengineering and biotechnology · 2021Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Academic research on dinoflagellate, the primary causative agent of harmful algal blooms (HABs), is often hindered by the coexistence with bacteria in laboratory cultures. The development of axenic dinoflagellate cultures is challenging and no universally accepted method suit for different algal species. In this study, we demonstrated a promising approach combined density gradient centrifugation, antibiotic treatment, and serial dilution to generate axenic cultures of Karenia mikimotoi (KMHK). Density gradient centrifugation and antibiotic treatments reduced the bacterial population from 5.79 ± 0.22 log
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