ArticleMicrobiologyOpen2021
Characteristics of DNA polymerase I from an extreme thermophile, Thermus scotoductus strain K1.
Article in MicrobiologyOpen, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 12 citations in OpenAlex.
- The critical role of diet, exercise, and sleep in shaping the gut microbiota of children with idiopathic short stature: a Retrospective study.Frontiers in immunology · 2025Article
- Characterization and PCR Application of Family B DNA Polymerases fromLife (Basel, Switzerland) · 2024Article
- Growth characteristics, redox potential changes and proton motive force generation inAIMS microbiology · 2024Article
- Mining thermophiles for biotechnologically relevant enzymes: evaluating the potential of European and Caucasian hot springs.Extremophiles : life under extreme conditions · 2023Review
- Thermophilic Nucleic Acid Polymerases and Their Application in Xenobiology.International journal of molecular sciences · 2022Review
- Optimized quantitative PCR analysis of random DNA aptamer libraries.Analytical biochemistry · 2022Article
- Microbial Diversity of Terrestrial Geothermal Springs in Armenia and Nagorno-Karabakh: A Review.Microorganisms · 2021Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several native and engineered heat-stable DNA polymerases from a variety of sources are used as powerful tools in different molecular techniques, including polymerase chain reaction, medical diagnostics, DNA sequencing, biological diversity assessments, and in vitro mutagenesis. The DNA polymerase from the extreme thermophile, Thermus scotoductus strain K1, (TsK1) was expressed in Escherichia coli, purified, and characterized. This enzyme belongs to a distinct phylogenetic clade, different from the commonly used DNA polymerase I enzymes, including those from Thermus aquaticus and Thermus thermophilus. The enzyme demonstrated an optimal temperature and pH value of 72-74°C and 9.0, respectively, and could efficiently amplify 2.5 kb DNA products. TsK1 DNA polymerase did not require additional K
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Registered trials
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