Evidence map›Paper›PMID 33415108›Full record

ArticleFrontiers in cell and developmental biology2020

Transfer of MicroRNA-216a-5p From Exosomes Secreted by Human Urine-Derived Stem Cells Reduces Renal Ischemia/Reperfusion Injury.

Yinmei Zhang, Junxiong Wang, Boxin Yang, Rui Qiao, Aiwei Li, Han Guo, Jie Ding, Hui Li, Hong Ye, Di Wu and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cell and developmental biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed, 1 pooled it
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Yinmei ZhangDepartment of Laboratory Medicine, Peking University Third Hospital, Beijing, China.
Junxiong WangDepartment of Gastroenterology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
Boxin YangDepartment of Laboratory Medicine, Peking University Third Hospital, Beijing, China.
Rui QiaoDepartment of Laboratory Medicine, Peking University Third Hospital, Beijing, China.
Aiwei LiDepartment of Laboratory Medicine, Peking University Third Hospital, Beijing, China.
Han GuoDepartment of Laboratory Medicine, Peking University Third Hospital, Beijing, China.
Jie DingDepartment of Laboratory Medicine, Peking University Third Hospital, Beijing, China.
Hui LiDepartment of Laboratory Medicine, Peking University Third Hospital, Beijing, China.
Hong YeDepartment of Laboratory Medicine, Peking University Third Hospital, Beijing, China.
Di WuDepartment of Laboratory Medicine, Peking University Third Hospital, Beijing, China.
Liyan CuiDepartment of Laboratory Medicine, Peking University Third Hospital, Beijing, China.
Shuo YangDepartment of Laboratory Medicine, Peking University Third Hospital, Beijing, China.
Peking University Third Hospital · CNPeking University · CNBeijing Friendship Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human urine-derived stem cells (USCs) protect rats against kidney ischemia/reperfusion (I/R) injury. Here we investigated the role of USCs exosomes (USCs-Exos) in protecting tubular endothelial cells and miRNA transfer in the kidney. Human USCs and USCs-Exos were isolated and verified by morphology and specific biomarkers. USC-Exos played a protective role in human proximal tubular epithelial cells (HK-2) exposed to hypoxia/reoxygenation (H/R). USCs-Exos were rich in miR-216a-5p, which targeted phosphatase and tensin homolog (PTEN) and regulated cell apoptosis through the Akt pathway. In HK-2 cells exposed to H/R, incubation with USC-Exos increased miR-216-5p, decreased PTEN levels, and stimulated Akt phosphorylation. Exposure of hypoxic HK-2 cells to USCs-Exos pretreated with anti-miR-216a-5p can prevent the increase of miR-216-5p and Akt phosphorylation levels, restore PTEN expression, and promote apoptosis. The dual-luciferase reported gene assay in HK-2 cells confirmed that miR-216a-5p targeted PTEN. In rats with I/R injury, intravenous infusion of USCs-Exos can effectively induce apoptosis suppression and functional protection, which is associated with decreased PTEN. Infusion of exosomes from anti-miR-216a-5p-transfected USCs weakened the protective effect in the I/R model. Therefore, USCs-Exos can reduce renal I/R injury by transferring miR-216a-5p targeting PTEN. Potentially, USCs-Exos rich in miR-216a-5p can serve as a promising therapeutic option for AKI.

Indexed as

acute kidney injuryexosomeshuman urine-derived stem cellsmiR-216a-5pphosphatase and tensin homolog

Identifiers

PMID33415108
PMCPMC7783217
OpenAlexW3116799174

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.