ArticleStem cell research & therapy2021
Establishing a deeper understanding of the osteogenic differentiation of monolayer cultured human pluripotent stem cells using novel and detailed analyses.
Article in Stem cell research & therapy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Influence of the addition of nanohydroxyapatite to scaffolds on proliferation and differentiation of human mesenchymal stem cells: a systematic review of in vitro studies.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2024Pooled it
- Preliminary Formulation-Dependent Angiogenesis-Related and Early Osteogenic Responses to Three-Dimensional Bioprinted Hydroxyapatite-Acrylated Palm Olein Scaffolds: An In Vitro Study.International journal of molecular sciences · 2026Article
- Mitochondrial Abundant Heat Soluble (MAHS) Protein Expression Modulates Metabolic Dynamics in Human Adipose-Derived Stem Cells.International journal of molecular sciences · 2026Article
- Leveraging Viscosity to Unlock the Osteogenic Potential of BMP-2 Mimetic DWIVA.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- A multifunctional conductive physiomimetic scaffold: synergy of rGO coating and cannabis-derived nanotopography for infection-resistant bone repair.Frontiers in bioengineering and biotechnology · 2026Article
- High-throughput quantification of osteogenesis in WJ-MSCs using Alizarin Red S, von Kossa, and Picro-Sirius red.Future science OA · 2025Article
- Hydrostatic Pressure Induces Osteogenic Differentiation of Single Stem Cells in 3D Viscoelastic Microgels.Small science · 2025Article
- Advancing Scaffold Architecture for Bone Tissue Engineering: A Comparative Study of 3D-Printed β-TCP Constructs in Dynamic Culture with pBMSC.Journal of functional biomaterials · 2025Article
- Development of a BMU-on-a-chip model based on spatiotemporal regulation of cellular interactions in the bone remodeling cycle.Materials today. Bio · 2025Article
- Synthesis and Osteoinductive Properties of Nanosized Lithium-Modified Calcium-Organic Frameworks.Materials (Basel, Switzerland) · 2025Article
- Moringa oleifera L. Nanosuspension Extract Administration Affects Heat Shock Protein-10 and -70 under Orthodontics Mechanical Force In Vivo.European journal of dentistry · 2025Article
- Donor age and breed determine mesenchymal stromal cell characteristics.Stem cell research & therapy · 2025Article
- Unraveling the Mechanism of Impaired Osteogenic Differentiation in Osteoporosis: Insights fromCells · 2024Article
- Antioxidant 1,2,3,4,6-Penta-Molecules (Basel, Switzerland) · 2024Article
- Single-layer graphene oxide nanosheets induce proliferation and Osteogenesis of single-cell hBMSCs encapsulated in Alginate Microgels.Scientific reports · 2024Article
- Correction to "Nanofibrous Microspheres: A Biomimetic Platform for Bone Tissue Regeneration".ACS applied bio materials · 2024Article
- Nanofibrous Microspheres: A Biomimetic Platform for Bone Tissue Regeneration.ACS applied bio materials · 2024Review
- Nanobioactive Blood-Derived Shear-Thinning Biomaterial for Tissue Engineering Applications.Applied materials today · 2024Article
- Design of Multi-Functional Bio-Safe Dental Resin Composites with Mineralization and Anti-Biofilm Properties.Journal of functional biomaterials · 2024Article
- Chitosan/Hyaluronan and Alginate-Nanohydroxyapatite Biphasic Scaffold as a Promising Matrix for Osteoarthritis Disorders.Advanced pharmaceutical bulletin · 2024Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDerivation of osteoblast-like cells from human pluripotent stem cells (hPSCs) is a popular topic in bone tissue engineering. Although many improvements have been achieved, the low induction efficiency because of spontaneous differentiation hampers their applications. To solve this problem, a detailed understanding of the osteogenic differentiation process of hPSCs is urgently needed.
methodsMonolayer cultured human embryonic stem cells and human-induced pluripotent stem cells were differentiated in commonly applied serum-containing osteogenic medium for 35 days. In addition to traditional assays such as cell viability detection, reverse transcription-polymerase chain reaction, immunofluorescence, and alizarin red staining, we also applied studies of cell counting, cell telomerase activity, and flow cytometry as essential indicators to analyse the cell type changes in each week.
resultsThe population of differentiated cells was quite heterogeneous throughout the 35 days of induction. Then, cell telomerase activity and cell cycle analyses have value in evaluating the cell type and tumourigenicity of the obtained cells. Finally, a dynamic map was made to integrate the analysis of these results during osteogenic differentiation of hPSCs, and the cell types at defined stages were concluded.
conclusionsOur results lay the foundation to improve the in vitro osteogenic differentiation efficiency of hPSCs by supplementing with functional compounds at the desired stage, and then establishing a stepwise induction system in the future.
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