ArticleBMC surgery2021
Identification of population of bacteria from culture negative surgical site infection patients using molecular tool.
Article in BMC surgery, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Prevalence of Anaerobic Bacteria in Surgical Site Infections in a Tertiary Care Hospital.Cureus · 2026Article
- Comparative diagnostic evaluation of real-time PCR and culture for detecting pathogens in podiatric wound infections.Microbiology spectrum · 2026Article
- Two cases of severe incision infection after cesarean section: a case report.Frontiers in medicine · 2026Article
- Current landscape and challenges in anaerobic bloodstream infections: epidemiology, microbiology, and clinical management.Frontiers in microbiology · 2026Review
- The Paradox of Healthcare in the 'Superbugs' Era: Current Challenges and Future Directions.Pathogens (Basel, Switzerland) · 2025Review
- Mortality Associated with Surgical Site Infections Following Cardiac Surgery: Insights from the International ID-IRI Study.IJID regions · 2025Article
- Viable but non-cultivable state in oral microbiota: a critical review of an underexplored microbial survival strategy.Frontiers in cellular and infection microbiology · 2025Review
- Predictors for prolonged and escalated perioperative antibiotic therapy after microvascular head and neck reconstruction: a comprehensive analysis of 446 cases.Head & face medicine · 2024Article
- Surgical Site Infection after Primary Open Surgery for Laryngeal Cancer in a Tertiary Hospital in Belgrade, Serbia: A 10-Year Prospective Cohort Study.Antibiotics (Basel, Switzerland) · 2024Article
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- Intraabdominal abscess mimicking gastric cancer recurrence: a case report.Journal of Yeungnam medical science · 2023Article
- The Diagnostic Value of Blood Next-Generation Sequencing in Early Surgical Site Infection After Spine Surgery.International journal of general medicine · 2023Article
- Surgical site infections by atypical mycobacteria: prevalence and species characterization using MALDI-TOF and molecular LCD chip array.Infection · 2022Article
- Combinatorial Therapeutic Strategy of Biogenics Derived from Lactobacillus fermentum PUM and Zingerone Against Pseudomonas aeruginosa PAO1-Induced Surgical Site Infection: an Experimental Study.Probiotics and antimicrobial proteins · 2022Article
- Article
- The Association of Gut Microbiota and Complications in Gastrointestinal-Cancer Therapies.Biomedicines · 2021Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundManaging surgical site infections, with negative culture report in routine diagnosis is a common dilemma in microbiology accounting more than 30% worldwide. The present study attempted to identify the presence of bacterial spp. if any in wound aspirates/swabs of culture negative surgical site infections of hospitalised patients using molecular tools.
methodsNinety-seven patients with post-operative SSI whose wound swabs/aspirate were negative in the conventional aerobic culture after 72 h of incubation were analysed by 16S rRNA gene specific broad range PCR. The amplified DNA fragments were sequenced by Sanger DNA sequencing method and homology of the sequence were matched using NCBI BLAST (NCBI, USA)
resultsOf the 97 patients, 16S rRNA based broad range PCR assay could identify the presence of bacterial pathogen in 53(54.63%) cases, of which 29 isolates were supposed to be of viable but non-culturable bacteria (VBNC), 07 were of obligatory anaerobes and 13 were of unculturable bacteria, 04 were with poly bacterial infections.
conclusionsOur study highlights the usefulness of PCR assay in detecting the presence of any VBNC, anaerobes and unculturable bacteria in SSI patients regardless of how well the bacteria may or may not grow in culture. Measures should be taken to use anaerobic culture system and PCR diagnosis along with conventional culture to detect the VBNC and unculturable bacteria where Gram stain is positive for better patient care.
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