ArticlePloS one2021
Reproducibility and normalization of reactive hyperemia using laser speckle contrast imaging.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Time-dependent microvascular and analgesic responses to cold compression in healthy adults: identifying the optimal cooling duration for recovery.European journal of applied physiology · 2026Trial
- Effect of ischemic preconditioning on microvascular post-occlusive hyperemic reactions and quadriceps morphology in MMA: a randomized clinical trial.European journal of applied physiology · 2026Trial
- Comparative assessment of healthy tissue oxygenation using near-infrared imaging, transcutaneous oxygen measurement, and plethysmography.Scientific reports · 2025Trial
- The role of the urologist in managing high flow priapism.International journal of impotence research · 2026Review
- Reactive Hyperemia Reveals Fractal Scaling and Multiscale Complexity in Photoplethysmography Waveforms.Biology · 2026Article
- Influence of Time of Day on Skin Microvascular Response to Post-Occlusive Reactive Hyperemia.Journal of clinical medicine · 2026Article
- Insulin Resistance is Associated With Skin Microvascular Dysfunction and Reduced Circulating Endothelial Progenitor Cells in Hypertensive Patients.Journal of clinical hypertension (Greenwich, Conn.) · 2026Article
- Optimizing In Vivo Perfusion Assessment by Laser Doppler Flowmetry-Effects of Probe Geometry and Signal Normalization.Diagnostics (Basel, Switzerland) · 2026Article
- A controlled comparative study on the effect of arterial occlusion pressure on immediate sympathetic and hyperemic responses.Scientific reports · 2025Article
- Peripheral Microvascular and Cerebral White Matter Dysfunction in Schizophrenia: Implications of a Body-Brain Endothelial Pathophysiology.Schizophrenia bulletin · 2025Article
- Article
- Influence of body position on microcirculatory and autonomic responses during arterial occlusion in healthy adults.PloS one · 2025Article
- Pressure-Induced Microvascular Reactivity With Whole Foot Loading Is Unique Across the Human Foot Sole.Microcirculation (New York, N.Y. : 1994) · 2025Article
- Methods of Quantitative Assessment of the Response of Dilated Skin Blood Vessels to High-Energy Light Treatments.Journal of clinical medicine · 2024Article
- Cardiac involved and autopsy in two patients with systemic sclerosis: Two cases report.Heliyon · 2023Article
- Spectral analysis of laser speckle contrast imaging and infrared thermography to assess skin microvascular reactive hyperemia.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2023Article
- Contactless photoplethysmography for assessment of small fiber neuropathy.Frontiers in physiology · 2023Article
- Characterizing tourniquet induced hemodynamics during total knee arthroplasty using diffuse optical spectroscopy.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2023Observational
- Correlation between Microvascular Damage and Internal Organ Involvement in Scleroderma: Focus on Lung Damage and Endothelial Dysfunction.Diagnostics (Basel, Switzerland) · 2022Review
- Laser Speckle Contrast Analysis: Functional Evaluation of Microvascular Damage in Connective Tissue Diseases. Is There Evidence of Correlations With Organ Involvement, Such as Pulmonary Damage?Frontiers in physiology · 2021Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundImpaired perfusion indices signal potential microvascular dysfunction preceding atherosclerosis and other cardiometabolic pathologies. Post-occlusive reactive hyperemia (PORH), a vasodilatory response following a mechanically induced ischemia, is a transient increase in perfusion and can assess microvascular function. The greatest blood flow change corresponding to the first minute of hyperemia (represented by time-to-peak, hyperemic velocity, AUC within 1st min) has been shown to indicate microvascular dysfunction. However, the reproducibility of these temporal kinetic indices of the PORH response is unknown. Our aim was to examine the inter- and intra-day reproducibility and standardization of reactive hyperemia, with emphasis on the kinetic indices of PORH, using laser speckle contrast imaging (LSCI) technique. METHODS AND
resultsSeventeen healthy adults (age = 24 ± 3 years) completed three PORH bouts over two lab visits. LSCI region of interest was a standardized 10 cm region on the dominant ventral forearm. A 5-min brachial artery occlusion period induced by inflating an arm cuff to 200 mmHg, preceded a 4-min hyperemic period. Inter- and intra-day reliability and reproducibility of cutaneous vascular conductance (LSCI flux / mean arterial pressure) were determined using intraclass correlation (ICC) and coefficient of variation (CV%). Maximal flow and area under the curve standardized to zero perfusion showed intra- and inter-day reliability (ICC > 0.70). Time to maximal flow (TMF) was not reproducible (inter-day CV = 18%). However, alternative kinetic indices such as 1-min AUC and overshoot rate-of-change (ORC), represented as a piecewise function (at 5s, 10s, 15s, and 20s into hyperemia), were reproducible (CV< 11%). Biological zero was a reliable normalization point.
conclusionPORH measured with LSCI is a reliable assessment of microvascular function. However, TMF or its derived hyperemic velocity are not recommended for longitudinal assessment. Piecewise ORC and 1-min AUC are reliable alternatives to assess the kinetic response of PORH.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.