ArticleACS nano2021
High-Speed Nanomechanical Mapping of the Early Stages of Collagen Growth by Bimodal Force Microscopy.
Article in ACS nano, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Collagen fibril diameter quantification using interference confocal reflectance microscopy.Biomedical optics express · 2026Article
- Real-time visualization of collagen assembly uncovers metastable properties in hierarchical organization.Nature communications · 2026Article
- Atomic Force Microscopy Investigations of Collagen: From Single Molecules to Fibrils.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Atomic Force Microscopy for Cross-Disciplinary Materials Research.Small methods · 2025Review
- Advances in nanomechanical property mapping by atomic force microscopy.Nanoscale advances · 2025Review
- High-Speed Quantitative Nanomechanical Mapping by Photothermal Off-Resonance Atomic Force Microscopy.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Altering the mechanical properties of self-assembled filaments through engineering of EspA bacterial protein.Materials today. Bio · 2025Article
- Revealing the Solution Conformation and Hydration Structure of Type I Tropocollagen Using X-ray Scattering and Molecular Dynamics Simulation.Biomacromolecules · 2025Article
- Real-time single-molecule observation of incipient collagen fibrillogenesis and remodeling.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- In Operando Imaging Electrostatic-Driven Disassembly and Reassembly of Collagen Nanostructures.ACS nano · 2024Article
- Article
- Light-induced modulation of viscoelastic properties in azobenzene polymers.Nanophotonics (Berlin, Germany) · 2024Article
- Technical advances in high-speed atomic force microscopy.Biophysical reviews · 2023Review
- Insights into Mechanical Dynamics of Nanoscale Interfaces in Epoxy Composites Using Nanorheology Atomic Force Microscopy.ACS applied materials & interfaces · 2023Article
- Sensing Dynamically Evolved Short-Range Nanomechanical Forces in Fast-Mutating Single Viral Spike Proteins.Small science · 2023Article
- Effect of Mechanical Microenvironment on Collagen Self-Assembly In Vitro.Journal of functional biomaterials · 2023Article
- Preparation, Characterization and ex vivo Skin Permeability Evaluation of Type I Collagen-Loaded Liposomes.International journal of nanomedicine · 2023Article
- Collagen piezoelectricity in osteogenesis imperfecta and its role in intrafibrillar mineralization.Communications biology · 2022Article
- Assessing Collagen D-Band Periodicity with Atomic Force Microscopy.Materials (Basel, Switzerland) · 2022Review
- Atomic Force Microscopy Nanomechanics of Hard Nanometer-Thick Films on Soft Substrates: Insights into Stretchable Conductors.ACS applied nano materials · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-speed atomic force microscopy (AFM) enabled the imaging of protein interactions with millisecond time resolutions (10 fps). However, the acquisition of nanomechanical maps of proteins is about 100 times slower. Here, we developed a high-speed bimodal AFM that provided high-spatial resolution maps of the elastic modulus, the loss tangent, and the topography at imaging rates of 5 fps. The microscope was applied to identify the initial stages of the self-assembly of the collagen structures. By following the changes in the physical properties, we identified four stages, nucleation and growth of collagen precursors, formation of tropocollagen molecules, assembly of tropocollagens into microfibrils, and alignment of microfibrils to generate microribbons. Some emerging collagen structures never matured, and after an existence of several seconds, they disappeared into the solution. The elastic modulus of a microfibril (∼4 MPa) implied very small stiffness (∼3 × 10
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.