ArticlePloS one2021
Rapid detection of Clostridium perfringens in food by loop-mediated isothermal amplification combined with a lateral flow biosensor.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Suboptimal PAM Enables One-Pot RPA-CRISPR/Cas12a Detection ofCurrent issues in molecular biology · 2026Article
- Prevalence of epsilon toxin-producing Clostridium perfringens isolates among patients with multiple sclerosis and neuromyelitis optica spectrum disorder in Iran.BMC neurology · 2025Article
- Conventional and molecular identification of Iranian Clostridia species associated with animal infection.Veterinary research forum : an international quarterly journal · 2025Article
- Revolutionizing food safety with electrochemical biosensors for rapid and portable pathogen detection.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Review
- Enhancing food safety in soybean fermentation through strategic implementation of starter cultures.Heliyon · 2024Review
- Article
- Recombinase polymerase amplification combined with lateral flow biosensor for rapid visual detection ofFrontiers in veterinary science · 2024Article
- Conventional and advanced detection techniques of foodborne pathogens: A comprehensive review.Heliyon · 2023Article
- Detection ofTransboundary and emerging diseases · 2023Article
- Dual-mode sensor based on the synergy of magnetic separation and functionalized probes for the ultrasensitive detection ofRSC advances · 2022Article
- Molecular Diagnostic Tools Applied for Assessing Microbial Water Quality.International journal of environmental research and public health · 2022Review
- eDNA-based detection of the invasive crayfish Pacifastacus leniusculus in streams with a LAMP assay using dependent replicates to gain higher sensitivity.Scientific reports · 2022Article
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Authors and funding
9 authors.
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Abstract
Clostridium perfringens is a key anaerobic pathogen causing food poisoning. Definitive detection by standard culture method is time-consuming and labor intensive. Current rapid commercial test kits are prohibitively expensive. It is thus necessary to develop rapid and cost-effective detection tool. Here, loop-mediated isothermal amplification (LAMP) in combination with a lateral-flow biosensor (LFB) was developed for visual inspection of C. perfringens-specific cpa gene. The specificity of the developed test was evaluated against 40 C. perfringens and 35 other bacterial strains, which showed no cross-reactivity, indicating 100% inclusivity and exclusivity. LAMP-LFB detection limit for artificially contaminated samples after enrichment for 16 h was 1-10 CFU/g sample, which was comparable to the commercial real-time PCR kit. The detection performance of LAMP-LFB was also compared to culture-based method using 95 food samples, which revealed the sensitivity (SE), specificity (SP) and Cohen's kappa coefficient (κ) of 88.0% (95% CI, 75.6%-95.4%), 95.5% (95% CI, 84.8%-99.4%) and 0.832 (95% CI, 0.721-0.943), respectively. Area under the receiver operating characteristic (ROC) curve was 0.918 (95% CI, 0.854-0.981), indicating LAMP-LFB as high relative accuracy test. In conclusion, LAMP-LFB assay is a low-cost qualitative method and easily available for routine detection of C. perfringens in food samples, which could serve as an alternative to commercial test kit.
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