ArticleApplied biochemistry and biotechnology2021
Identification and Characterization of an Efficient Phenylalanine Ammonia-Lyase from Photorhabdus luminescens.
Article in Applied biochemistry and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 16 citations in OpenAlex.
- Estimation of Phenylalanine Ammonia-Lyase Activity for Assessing Resistance of Muskmelon Against Fusarium oxysporum f. sp. melonis Infection.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Phenylalanine Ammonia-Lyase: A Core Regulator of Plant Carbon Metabolic Flux Redistribution-From Molecular Mechanisms and Growth Modulation to Stress Adaptability.Plants (Basel, Switzerland) · 2025Review
- Screening and transcriptomic profiling of tobacco growth-promoting arbuscular mycorrhizal fungi.Plant signaling & behavior · 2025Article
- Microbial engineering for monocyclic aromatic compounds production.FEMS microbiology reviews · 2025Review
- Article
- In-depth Sequence-Function Characterization Reveals Multiple Pathways to Enhance Enzymatic Activity.ACS catalysis · 2022Article
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
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Abstract
A putative aromatic amino acid ammonia-lyase gene (named Pl-pal) was discovered in Photorhabdus luminescens DSM 3368. BLAST and phylogenetic analyses predicted that this enzyme is a histidine ammonia-lyase, whereas sequence alignment suggested that it is more likely a phenylalanine ammonia-lyase (PAL). This gene was amplified from P. luminescens and expressed in Escherichia coli BL21(DE3). The function of Pl-PAL (58 kDa) was characterized by in vitro enzymatic reactions with L-phenylalanine (L-Phe), L-tyrosine (L-Tyr), L-histidine (L-His), and L-tryptophan (L-Trp). Pl-PAL can convert L-Phe and L-Tyr to trans-cinnamic acid and p-coumaric acid, respectively, but had no function on L-His and L-Trp. The optimum temperature and pH were determined to be 40 °C and 11.0, respectively. Under the optimal conditions, Pl-PAL had a k
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