Evidence map›Paper›PMID 33409934›Full record

ArticleJournal of applied genetics2021

Cytomolecular analysis of mutants, breeding lines, and varieties of camelina (Camelina sativa L. Crantz).

Michał T Kwiatek, Zofia Drozdowska, Danuta Kurasiak-Popowska, Aleksandra Noweiska, Jerzy Nawracała

Abstract read
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In one paragraph

Article in Journal of applied genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Overcoming genetic paucity ofFrontiers in plant science · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Michał T KwiatekDepartment of Genetics and Plant Breeding, Faculty of Agronomy and Bioengineering, Poznań University of Life Sciences, Dojazd 11Str., 60-632, Poznan, Poland. michal.kwiatek@up.poznan.pl.ORCID https://orcid.org/0000-0001-9442-3124
Zofia DrozdowskaDepartment of Genetics and Plant Breeding, Faculty of Agronomy and Bioengineering, Poznań University of Life Sciences, Dojazd 11Str., 60-632, Poznan, Poland.
Danuta Kurasiak-PopowskaDepartment of Genetics and Plant Breeding, Faculty of Agronomy and Bioengineering, Poznań University of Life Sciences, Dojazd 11Str., 60-632, Poznan, Poland.ORCID https://orcid.org/0000-0002-2214-406X
Aleksandra NoweiskaDepartment of Genetics and Plant Breeding, Faculty of Agronomy and Bioengineering, Poznań University of Life Sciences, Dojazd 11Str., 60-632, Poznan, Poland.
Jerzy NawracałaDepartment of Genetics and Plant Breeding, Faculty of Agronomy and Bioengineering, Poznań University of Life Sciences, Dojazd 11Str., 60-632, Poznan, Poland.ORCID https://orcid.org/0000-0002-6824-156X

Funding

Ministerstwo Nauki i Szkolnictwa Wyższego 005/RID/2018/19
6 · The paper itself

Abstract

Camelina sativa L. Crantz (Brassicaceae family), known as camelina, has gained new attention as a re-emerging oil seed crop. With a unique seed oil profile, with the majority of the fatty acids consisting of linolenic (C18:3), oleic (C18:1), linoleic (C18:2), and eicosenoic (C20:1), camelina oil is reported to be useful as a food oil and biofuel. However, there are still many unknown factors about the structure and genetic variability of this crop. Chromosomal localization of ribosomal DNA was performed using fluorescence in situ hybridization (FISH) with 5S rDNA and 25S rDNA sequences as molecular probes on mitotic chromosomes of enzymatically digested root-tip meristematic cells. Here, we present for the first time a comparative analysis of selected genotypes (cultivars, breeding lines and mutants) of C. sativa with the use of cytogenetic techniques. The main aim of the study was to determine the intraspecific and interspecific polymorphisms in the structure of chromosomes of selected accessions using conserved 5S and 25S rDNA repetitive sequences as molecular probes. The results were compared with C. microcarpa (closely related to C. sativa) rDNA gene loci distribution. The presence of minor rDNA sites was discussed and compared with other Brassicaceae species. In addition, demonstration karyograms of C. sativa and C. microcarpa mapped with rDNA probes were prepared based on the cv. "Przybrodzka" and GE2011-02 genotype, respectively. The use of 5S and 25S rDNA probes provided an insight on the genome structure of C. sativa at the cytogenetic level and can help to understand the genome organization of this crop. The putative role of cytogenetic markers in phylogenetic analyses of camelina was discussed, as well.

Indexed as

BrassicaceaePlant BreedingDNA ProbesGenome, PlantIn Situ Hybridization, FluorescencePhylogenyPlant OilsRNA, RibosomalRNA, Ribosomal, 5SDNA ProbesPlant OilsRNA, RibosomalRNA, ribosomal, 25SRNA, Ribosomal, 5SCamelina sativaFluorescence in situ hybridizationKaryogramMolecular cytogeneticsrDNA gene loci

Identifiers

PMID33409934

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.