ArticleBiomedical optics express2020
Deep-learning super-resolution light-sheet add-on microscopy (Deep-SLAM) for easy isotropic volumetric imaging of large biological specimens.
Article in Biomedical optics express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Technological innovations and high-throughput applications of light-sheet microscopy.Biophysics reports · 2026Article
- Adaptive-learning physics-assisted light-field microscopy enables day-long and millisecond-scale super-resolution imaging of 3D subcellular dynamics.Nature communications · 2025Article
- Optical sectioning methods in three-dimensional bioimaging.Light, science & applications · 2025Review
- Three-dimensional random-access confocal microscopy with 3D remote focusing system.Communications engineering · 2024Article
- Applications of Lightsheet Fluorescence Microscopy by High Numerical Aperture Detection Lens.The journal of physical chemistry. B · 2024Review
- Connecto-informatics at the mesoscale: current advances in image processing and analysis for mapping the brain connectivity.Brain informatics · 2024Review
- Deep learning methods for high-resolution microscale light field image reconstruction: a survey.Frontiers in bioengineering and biotechnology · 2024Review
- Rapid 3D isotropic imaging of whole organ with double-ring light-sheet microscopy and self-learning side-lobe elimination.Biomedical optics express · 2023Article
- Roadmap on Label-Free Super-Resolution Imaging.Laser & photonics reviews · 2023Article
- Nondestructive 3D Pathology with Light-Sheet Fluorescence Microscopy for Translational Research and Clinical Assays.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- Challenges and advances in optical 3D mesoscale imaging.Journal of microscopy · 2022Article
- Elimination of stripe artifacts in light sheet fluorescence microscopy using an attention-based residual neural network.Biomedical optics express · 2022Article
- Light Sheet Illumination for 3D Single-Molecule Super-Resolution Imaging of Neuronal Synapses.Frontiers in synaptic neuroscience · 2021Review
- Navigating the Light-Sheet Image Analysis Software Landscape: Concepts for Driving Cohesion From Data Acquisition to Analysis.Frontiers in cell and developmental biology · 2021Review
Corrections and comments
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Authors and funding
6 authors.
Funding
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Abstract
Isotropic 3D histological imaging of large biological specimens is highly desired but remains highly challenging to current fluorescence microscopy technique. Here we present a new method, termed deep-learning super-resolution light-sheet add-on microscopy (Deep-SLAM), to enable fast, isotropic light-sheet fluorescence imaging on a conventional wide-field microscope. After integrating a minimized add-on device that transforms an inverted microscope into a 3D light-sheet microscope, we further integrate a deep neural network (DNN) procedure to quickly restore the ambiguous z-reconstructed planes that suffer from still insufficient axial resolution of light-sheet illumination, thereby achieving isotropic 3D imaging of thick biological specimens at single-cell resolution. We apply this easy and cost-effective Deep-SLAM approach to the anatomical imaging of single neurons in a meso-scale mouse brain, demonstrating its potential for readily converting commonly-used commercialized 2D microscopes to high-throughput 3D imaging, which is previously exclusive for high-end microscopy implementations.
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Registered trials
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