Evidence map›Paper›PMID 33408991›Full record

ArticleBiomedical optics express2020

Dual-view oblique plane microscopy (dOPM).

Hugh Sparks, Lucas Dent, Chris Bakal, Axel Behrens, Guillaume Salbreux, Chris Dunsby

Open access · goldAbstract read
In one paragraph

Article in Biomedical optics express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
7.2field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 49 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Recent advances in oblique plane microscopy.Nanophotonics (Berlin, Germany) · 2023
    Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Hugh SparksPhotonics Group, Department of Physics, Imperial College London, London, SW7 2AZ, UK.ORCID https://orcid.org/0000-0001-9900-6610
Lucas DentDynamical Cell Systems Team, The Institute of Cancer Research, London, SW3 6JB, UK.
Chris BakalDynamical Cell Systems Team, The Institute of Cancer Research, London, SW3 6JB, UK.
Axel BehrensCancer Stem Cell Team, The Institute of Cancer Research, London, SW3 6JB, UK.
Guillaume SalbreuxThe Theoretical Physics of Biology Laboratory, The Francis Crick Institute, London, NW1 1AT, UK.
Chris DunsbyPhotonics Group, Department of Physics, Imperial College London, London, SW7 2AZ, UK.
Institute of Cancer Research · GBImperial College London · GBThe Francis Crick Institute · GB

Funding

Cancer Research UK 20146Cancer Research UK 21144Wellcome Trust FC001039Wellcome Trust FC001317
6 · The paper itself

Abstract

We present a new folded dual-view oblique plane microscopy (OPM) technique termed dOPM that enables two orthogonal views of the sample to be obtained by translating a pair of tilted mirrors in refocussing space. Using a water immersion 40× 1.15 NA primary objective, deconvolved image volumes of 200 nm beads were measured to have full width at half maxima (FWHM) of 0.35 ± 0.04 µm and 0.39 ± 0.02 µm laterally and 0.81 ± 0.07 µm axially. The measured z-sectioning value was 1.33 ± 0.45 µm using light-sheet FWHM in the frames of the two views of 4.99 ± 0.58 µm and 4.89 ± 0.63 µm. To qualitatively demonstrate that the system can reduce shadow artefacts while providing a more isotropic resolution, a multi-cellular spheroid approximately 100 µm in diameter was imaged.

Identifiers

PMID33408991
PMCPMC7747899
OpenAlexW3097743579

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.