Evidence map›Paper›PMID 33408404›Full record

ArticleNature methods2021

Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging.

Weijian Zong, Runlong Wu, Shiyuan Chen, Junjie Wu, Hanbin Wang, Zhe Zhao, Guoqing Chen, Rui Tu, Danlei Wu, Yanhui Hu and 9 more

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Nature methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 101 papers.

0numbers the graph read from it
0cells of the map it votes in
101citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

101 citing papers in PubMed.

  1. Article
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  8. Review
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  10. A dual-color miniature endoscope for calcium imaging in behaving mice.bioRxiv : the preprint server for biology · 2026
    Article
  11. Article
  12. Article
  13. Review
  14. Article
  15. Article
  16. Article
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  19. Miniaturized widefield microscope for high speed in vivo voltage imaging.bioRxiv : the preprint server for biology · 2025
    Article
  20. Article

41 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

19 authors.

Weijian Zong *State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, College of Future Technology, Peking University, Beijing, China.
Runlong Wu *State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, College of Future Technology, Peking University, Beijing, China. rlwu@pku.edu.cn.ORCID http://orcid.org/0000-0002-8084-3010
Shiyuan ChenState Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, College of Future Technology, Peking University, Beijing, China.
Junjie WuCollege of Engineering, Peking University, Beijing, China.
Hanbin WangAcademy of Advanced Interdisciplinary Study, Peking University, Beijing, China.
Zhe ZhaoDepartment of Neurobiology, Institute of Basic Medical Sciences, Beijing, China.
Guoqing ChenState Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, College of Future Technology, Peking University, Beijing, China.
Rui TuState Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, College of Future Technology, Peking University, Beijing, China.
Danlei WuBeijing Transcend Vivoscope Biotech Co., Ltd, Beijing, China.
Yanhui HuBeijing Transcend Vivoscope Biotech Co., Ltd, Beijing, China.
Yangyang XuBeijing Transcend Vivoscope Biotech Co., Ltd, Beijing, China.
Yao WangAcademy of Advanced Interdisciplinary Study, Peking University, Beijing, China.
Zhuoli DuanDepartment of Neurology, Southern Medical University, Guangzhou, China.
Haitao WuDepartment of Neurobiology, Institute of Basic Medical Sciences, Beijing, China.ORCID http://orcid.org/0000-0001-8437-3194
Yunfeng ZhangDepartment of Electronics, Peking University, Beijing, China.ORCID http://orcid.org/0000-0003-2418-2120
Jue ZhangCollege of Engineering, Peking University, Beijing, China.ORCID http://orcid.org/0000-0003-0440-1357
Aimin WangDepartment of Electronics, Peking University, Beijing, China. wangaimin@pku.edu.cn.ORCID http://orcid.org/0000-0002-5484-9494
Liangyi ChenState Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, College of Future Technology, Peking University, Beijing, China. lychen@pku.edu.cn.ORCID http://orcid.org/0000-0003-1270-7321
Heping ChengState Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, College of Future Technology, Peking University, Beijing, China.ORCID http://orcid.org/0000-0002-9604-6702

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We have developed a miniature two-photon microscope equipped with an axial scanning mechanism and a long-working-distance miniature objective to enable multi-plane imaging over a volume of 420 × 420 × 180 μm

Indexed as

AnimalsBehavior, AnimalBrainCytological TechniquesLocomotionMaleMiceMice, Inbred C57BLMicroscopy, Fluorescence, MultiphotonMiniaturizationNeuroimaging

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.