Evidence map›Paper›PMID 33407952›Full record

ArticleJournal of biological research (Thessalonike, Greece)2021

Circ_0000396 inhibits rheumatoid arthritis synovial fibroblast growth and inflammatory response via miR-203/HBP1 axis.

Laifang Wang, Qing Zhao, Na Wang, Yanjie Ding, Lingli Kong, Jing Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biological research (Thessalonike, Greece), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 21 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Article
  6. Review
  7. Article
  8. Review
  9. Article
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Laifang WangDepartment of Rheumatism and Immunology, Huaihe Hospital of Henan University, No. 115 Ximen Street, Kaifeng, 475000, Henan, China.
Qing ZhaoDepartment of Rheumatism and Immunology, Huaihe Hospital of Henan University, No. 115 Ximen Street, Kaifeng, 475000, Henan, China.
Na WangDepartment of Rheumatism and Immunology, Huaihe Hospital of Henan University, No. 115 Ximen Street, Kaifeng, 475000, Henan, China.
Yanjie DingDepartment of Rheumatism and Immunology, Huaihe Hospital of Henan University, No. 115 Ximen Street, Kaifeng, 475000, Henan, China. sigtbs@163.com.
Lingli KongDepartment of Rheumatism and Immunology, Huaihe Hospital of Henan University, No. 115 Ximen Street, Kaifeng, 475000, Henan, China.
Jing WangDepartment of Rheumatism and Immunology, Huaihe Hospital of Henan University, No. 115 Ximen Street, Kaifeng, 475000, Henan, China.
Henan University Huaihe Hospital and Huaihe Clinical Institute · CN

Funding

Henan Province Medical Science and Technology Project Joint Development Project 2018020329Henan Province Traditional Chinese Medicine Scientific Research Project 2017ZY3044
6 · The paper itself

Abstract

backgroundCirc_0000396 was found to be down-regulated in the rheumatoid arthritis (RA) patients and had a high diagnostic value. However, the function and mechanisms underlying circ_0000396 in RA progression remain unclear.

methodsThe expression of circ_0000396, microRNA (miR)-203 and HMG-box transcription factor 1 (HBP1) was detected using qRT-PCR and western blot. The proliferative and apoptotic capabilities of rheumatoid arthritis synovial fibroblasts (RASFs) were measured by colony formation, CCK-8, flow cytometry and western blot assays, respectively. The levels of interleukins (IL)-6, IL-1β, IL-8 and tumor necrosis factor-α (TNF-α) were detected using enzyme-linked immunosorbent assay (ELISA). The target correlations between miR-203 and circ_0000396 or HBP1 were validated using pull-down and dual-luciferase reporter assay.

resultsCirc_0000396 was decreased in RA synovial tissues and RASFs, and overexpression of circ_0000396 suppressed cell proliferation, induced cell apoptosis and reduced the release of inflammatory cytokine IL-6, IL-1β, IL-8 and TNF-α in RASFs, while circ_0000396 deletion functioned oppositely. MiR-203 was confirmed to be a target of circ_0000396, and miR-203 reversed the protective effects of circ_0000396 on the dysfunction and inflammation of RASFs. HBP1 was a target of miR-203, and silencing miR-203 inhibited RASFs malignant changes by regulating HBP1. In addition, circ_0000396 could regulate HBP1 by sponging miR-203, and HBP1 decrease attenuated the effects of circ_0000396 on RASF growth and inflammation.

conclusionCirc_0000396 inhibited the growth and inflammation in RASFs by regulating miR-203/HBP1 axis, providing a potential therapeutic target for RA.

Indexed as

circ_0000396miR-203; HBP1Rheumatoid arthritisSynovial fibroblasts

Identifiers

PMID33407952
PMCPMC7788801
OpenAlexW3121089048

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.