ArticleJournal of orthopaedic surgery and research2021
Direct comparison of 3D and 2D cultivation reveals higher osteogenic capacity of elderly osteoblasts in 3D.
Article in Journal of orthopaedic surgery and research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
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- Coordination of Lipid Storage and Mobilization Pathways During Osteoblast Maturation in a 3D Human Bone Model.International journal of molecular sciences · 2026Article
- Establishment of a three-dimensional in vitro peri-implant bone-mucosa composite model.BMC oral health · 2026Article
- RGD Peptide-Based Hydrogel Enhances the Osteogenic Differentiation of Periodontal Ligament Stem CellsCurrent protein & peptide science · 2026Article
- Stem cell modelling of osteoarthritis using an organ-on-a-chip approach.Inflammation and regeneration · 2025Article
- Human platelet-derived extracellular vesicle fractions modulate bone cell metabolism and biologize volume-stable β-TCP matrix in vitro.BMC medicine · 2025Article
- Human bone marrow derived stem cell differentiation on 3D printed bioactive glass scaffolds.Journal of materials science. Materials in medicine · 2025Article
- Bioinspired 3D Bone Model: Mimicking the Cortical-Spongy Bone Architecture and Biology for Enhanced Physiological Representation of Bone.ACS biomaterials science & engineering · 2025Article
- Vitamin D Alleviates Cadmium-Induced Inhibition of Chicken Bone Marrow Stromal Cells' Osteogenic Differentiation In Vitro.Animals : an open access journal from MDPI · 2023Article
- Bioengineering extracellular vesicles: smart nanomaterials for bone regeneration.Journal of nanobiotechnology · 2023Review
- Bone mineralisation and glucose metabolism.Current opinion in endocrine and metabolic research · 2023Review
- Crypt-Villus Scaffold Architecture for Bioengineering Functional Human Intestinal Epithelium.ACS biomaterials science & engineering · 2022Article
- Article
- A critical review of in vitro research methodologies used to study mineralization in human dental pulp cell cultures.International endodontic journal · 2022Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe aim of this study was the investigation of the osteogenic potential of human osteoblasts of advanced donor age in 2D and 3D culture.
methodsOsteoblasts were induced to osteogenic differentiation and cultivated, using the same polystyrene material in 2D and 3D culture for 2 weeks. Samples were taken to evaluate alkaline phosphatase (ALP) activity, mineralization and gene expression.
resultsOsteoprotegerin (OPG) levels were significantly increased (8.2-fold) on day 7 in 3D compared to day 0 (p < 0.0001) and 11.6-fold higher in 3D than in 2D (p < 0.0001). Both culture systems showed reduced osteocalcin (OC) levels (2D 85% and 3D 50% of basic value). Collagen type 1 (Col1) expression was elevated in 3D on day 7 (1.4-fold; p = 0.009). Osteopontin (OP) expression showed 6.5-fold higher levels on day 7 (p = 0.002) in 3D than in 2D. Mineralization was significantly higher in 3D on day 14 (p = 0.0002).
conclusionAdvanced donor age human primary osteoblasts reveal significantly higher gene expression levels of OPG, Col1 and OP in 3D than in monolayer. Therefore, it seems that a relatively high potential of bone formation in a natural 3D arrangement is presumably still present in osteoblasts of elderly people.
trial registration5217/11 on the 22nd of Dec. 2011.
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