Evidence map›Paper›PMID 33407462›Full record

ArticleMicrobial cell factories2021

Heterologous expression of Phanerochaete chrysosporium cellobiose dehydrogenase in Trichoderma reesei.

Lena Wohlschlager, Florian Csarman, Hucheng Chang, Elisabeth Fitz, Bernhard Seiboth, Roland Ludwig

Open access · goldAbstract read
In one paragraph

Article in Microbial cell factories, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 26 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Lena WohlschlagerBiocatalysis and Biosensing Laboratory, Department of Food Science and Technology, BOKU-University of Natural Resources and Life Sciences, Muthgasse 18, 1190, Vienna, Austria.
Florian CsarmanBiocatalysis and Biosensing Laboratory, Department of Food Science and Technology, BOKU-University of Natural Resources and Life Sciences, Muthgasse 18, 1190, Vienna, Austria.
Hucheng ChangBiocatalysis and Biosensing Laboratory, Department of Food Science and Technology, BOKU-University of Natural Resources and Life Sciences, Muthgasse 18, 1190, Vienna, Austria.
Elisabeth FitzResearch Division Biochemical Technology, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, 1060, Vienna, Austria.
Bernhard SeibothResearch Division Biochemical Technology, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, 1060, Vienna, Austria.
Roland LudwigBiocatalysis and Biosensing Laboratory, Department of Food Science and Technology, BOKU-University of Natural Resources and Life Sciences, Muthgasse 18, 1190, Vienna, Austria. roland.ludwig@boku.ac.at.ORCID http://orcid.org/0000-0002-5058-5874
BOKU University · ATTU Wien · AT

Funding

European Research Council 726396
6 · The paper itself

Abstract

backgroundCellobiose dehydrogenase from Phanerochaete chrysosporium (PcCDH) is a key enzyme in lignocellulose depolymerization, biosensors and biofuel cells. For these applications, it should retain important molecular and catalytic properties when recombinantly expressed. While homologous expression is time-consuming and the prokaryote Escherichia coli is not suitable for expression of the two-domain flavocytochrome, the yeast Pichia pastoris is hyperglycosylating the enzyme. Fungal expression hosts like Aspergillus niger and Trichoderma reesei were successfully used to express CDH from the ascomycete Corynascus thermophilus. This study describes the expression of basidiomycetes PcCDH in T. reesei (PcCDH

resultsPcCDH

conclusionsHeterologous production of PcCDH

Indexed as

Carbohydrate DehydrogenasesCellobioseGlycosylationHypocrealesPhanerochaeteRecombinant ProteinsTransformation, GeneticCarbohydrate DehydrogenasesCellobiosecellobiose-quinone oxidoreductaseRecombinant ProteinsCatalytic constantsCellobiose dehydrogenaseCofactor occupancyGlycosylationLignocellulose depolymerizationPhanerochaete chrysosporiumRedox potentialTrichoderma reesei

Identifiers

PMID33407462
PMCPMC7789494
OpenAlexW3120428342

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.